1335 Artemisia capillaris flower extract protects hyaluronic acid from degradation by HYBID in human dermal fibroblasts

Hyaluronic acid/Hyaluronan (HA) is an important component of the extracellular matrix dermis. HYBID (HA-Binding protein Involved in HA Depolymerization), also known as KIAA1199/CEMIP, plays a key role degradation large (>1000 kDa) into intermediate size fragments with molecular weights 10 kDa to 100 kDa. Recently, we have demonstrated that Artemisia capillaris flower extract suppresses histamine-induced expression neonatal normal human dermal fibroblasts (NHDF). In this study, responsible for HA, evaluated effect same plant on protection from under experimental conditions. NHDF were maintained Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum. After cells treated different concentrations around 3 hrs, they exposed inducer, histamine, additional hrs followed by addition fluorescein isothiocyanate (FITC)-labeled (1,200-1,600 kDa). 24 and 48 incubation, cultured medium was collected measurement distribution FITC-labeled HPLC. It found not only decreased, but increased HYBID. contrast, use time- dose-dependently reversed action, which leads degradation. As loss has been reported be directly correlated wrinkle sagging skin, could provide non-invasive solution protect our skin against aging through