1272 p21Waf1/Cip1 is differentially expressed in epidermal versus follicular melanocytes and melanoma cells and is phenotypically regulated by UVB-mediated apoptosis

Melanoma is a malignant cancer of pigment-producing cells in our skin, exhibiting an exceptional mutational burden and drug resistance. This partly due to exposure UV radiation higher incidence genetic variations associated with fair freckles, red hair, which can lead DNA damage melanoma development. Defective apoptosis pathways constitute one barrier treatment. Thus, targeting regulators promising approach sensitizing melanomas for Using multiplex approach, we studied the expression markers primary human epidermal follicular melanocytes, (HEM, HFM), compared it selected cell lines variable melanin levels subtype (FM55P, FM55M, SK-MEL-23). A distinct profile was found between normal melanocytes (FM55P), metastatic (FM55M, SK-MEL-23) cells. In particular, cyclin-dependent kinase inhibitor p21 (p21WAF1/Cip1) be much more highly expressed HFM) than Western-blot analysis revealed highest bulb-derived (HFM), followed by HEM much-reduced lines. may relate unique traits hair follicle that naturally proliferate apoptose during growth cycle are remarkably resistant melanomagenesis. Ultraviolet (UVB) induces melanocyte death vitro upon UVB (300mJ/cm2) protein increased linked decreased Bcl2. suggests disruption repair processes controlled pigment way preventing progression.