1114 Study of long-term (15-day) imiquimod treatment and drug intervention in localized psoriasiform dermatitis model

Imiquimod (IMQ)-induced acute skin inflammation is the most widely used animal model in field of psoriasis research. Several information already available on effect IMQ dermatitis, but little known about its long-term application. Here, this study we tested treatment and an anti-inflammatory drug two mouse strains different experimental setups using localized psoriasiform dermatitis model. Aldara cream (5% IMQ) was applied Finn chamber dorsal C57BL/6J or Balb/c mice during first 4 days 15-day experiment every second third day afterwards separate groups. Vaseline corticosteroid ointment were intermediary days. Skin thickness, blood perfusion, body weight local PASI calculated daily. samples collected 5, 10 15 experiment. Histopathological alterations evaluated H&E stained sections, while inflammatory cytokines determined by qPCR Luminex technology. Clinical signs intensified Day 5 each groups mice, then regained normal phenotypical appearance. From 12 experiment, hair growth observed Aldara-treated areas group both strains, except steroid group. infiltration increased until 5th a peak developed from 10th end Topical significantly reduced 6 strains. Inflammatory after topical Our results proved that application chambers enables treatment. Furthermore, our setup may be to investigate topically candidates dermatitis.