(097) A Novel Therapeutic Strategy for Recovery from Cavernous Veno-Occlusive Dysfunction through Chronic Administration of Hepatocyte Growth Factor and a JNK Inhibitor in a Rat Model of Cavernous Nerve Injury

Abstract Introduction Structural alterations of penis including cavernosal apoptosis and fibrosis induce venous leakage in the corpus cavernosum or veno-occlusive dysfunction, a key pathophysiology erectile dysfunction (ED) after radical prostatectomy. Objective We aimed to determine if JNK inhibition combination with intracavernosal administration hepatocyte growth factor (HGF) would completely restore function (CVOF) rat model ED induced by bilateral CN crush injury (CNCI). Methods A total forty-two male Sprague-Dawley rats were randomly assigned sham control (S), CNCI (I) treated inhibitors HGF (J+H) for 5 weeks surgery. All each group evaluated dynamic infusion cavernosometry (DIC), caspase-3 activity assay, Masson’s trichrome staining, immunohistochemical staining α-smooth muscle actin (α-SMA) immunoblotting at Results Regarding CVOF, Group I showed decreased papaverine response, an increased maintenance drop rates compared S. J+H significant improvements three DIC parameters I. There no differences response rate between structural integrity cavernosum, had activity, SM/collagen ratio, content SM, protein expression PECAM-1, phosphorylation c-Jun c-Met. alleviation histological molecular derangement SM content, PECAM-1 expression, c-Met Conclusions Our data indicate that chronic may CVOF level comparable through preservation cavernosum. Combination might be potential therapeutic option recovering from CVOD. Disclosure No