061 Spatial proteomics and transcriptomics with digital spatial profiling reveals overlapping, but distinct inflammatory pathways in discoid lupus and lichen planus

Lichen planus (LP) and discoid lupus erythematosus (DLE) are chronic, inflammatory autoimmune skin diseases with interface dermatitis significant morbidity whose pathogenesis is incompletely understood. Recently, NanoString Technologies has developed GeoMx® digital spatial profiling (DSP), a novel highly multiplex method for detection of proteins RNA in fixed tissues. Here, we perform proteomics transcriptomics using DSP to further characterize immune activation immunoregulatory function, including checkpoints, these overlapping diseases. We utilized 58-marker antibody cocktail found that DLE LP express unique combination inhibitory receptors. For example, programmed death receptor ligand 1 (PD-L1) lymphocyte-activation gene 3 (LAG3) detected greater amounts than LP, while expresses more T-cell immunoglobulin mucin-domain containing-3 (TIM3). next used the Cancer Transcriptome Atlas (>1800 genes) on separate cohort patients LP. Within CD3 enriched areas, there were 17 differentially expressed genes IRF7, STAT2, TNFSF10 type I IFN (IFITM1, MX1, LY6E). In contrast, IFNA17 NK cell associated KIR2DL1. IFN-g related not significantly different, suggesting both have strong II signatures, an overall stronger signature addition TNF NF-kb signaling. There few limitations our study. Our study its conclusions limited by small discovery (n=6). As such, should be taken as preliminary demonstration how can generate insights into checkpoint expression pathway DLE.