006 IL-6 enhances IL-6 expression by epidermal langerhans cells (LCs)

Exposure of endothelial cells (ECs) to the neuropeptide calcitonin gene-related peptide (CGRP) endows them with ability, as bystanders, bias outcome LC antigen (Ag) presentation T toward Th17 pole and our previous work indicates that EC IL-6 production mediates most this effect. Preliminary experiments suggested could enhance expression by LCs. We have further examined effect released CGRP-exposed ECs may stimulate LCs produce additional IL-6, potentially involved in regulating Ag presentation. To examine impact on gene expression, purified BALB/c were exposed complete medium (CM) alone, 10 ng/ml or 100 for 3-hrs 6-hrs. Then, total RNA was extracted analysis RNAseq. exposure induced significant increases mRNA: 4.63-fold 6-hrs 4.16-fold ng/ml; 1.80-fold 2.15-fold 3 hrs ng/ml. determine if enhanced protein treated CM alone. After culture, washed 4 times placed containing GM-CSF (known viability) Supernatants harvested 24-hrs later concentration assessed ELISA. alone failed increase release into medium. However, at led a [118.5+/-6.8 (SEM) pg/ml vs. 27.1+/-6.6 CM]. combined substantially significantly increased [173.7+/-12.9 311.8+/-17.2 pg/ml, respectively, 6.8+/-0.8 alone]. Thus, presenting cultures greatly This ECs, be trans-presented responding consequent biasing immunity pole. cell products, such GM-CSF, IL-6.