منابع مشابه
Enzymatic synthesis of [3'-O-methyl-(3)H]malvidin-3-glucoside from petunidin-3-glucoside.
Malvidin-3-glucoside has been labeled by enzymatic synthesis in a single-step experiment. Catechol-O-methyl transferase catalyzed the B-ring methylation of petunidin-3-glucoside, and S-Adenosyl-L-[methyl-(3)H] methionine was the methyl donor. Solid phase extraction and preparative high-performance liquid chromatography were necessary to separate [3'-O-methyl-(3)H]malvidin-3-glucoside from an is...
متن کاملErythrocyte 3-O-methyl-D-glucose uptake assay for diagnosis of glucose-transporter-protein syndrome.
Glucose transport into the brain is mediated by a facilitative glucose-transporter protein, GLUT-1. A GLUT-1 defect results in the Glucose-Transporter-Protein Syndrome (GTPS), characterized by infantile epilepsy, developmental delay, and acquired microcephaly. The diagnosis is currently based on clinical features, low to normal lactate levels and low glucose levels (hypoglycorrhachia) in the ce...
متن کاملSynthesis and biologic evaluation of (11)c-methyl-d-glucoside, a tracer of the sodium-dependent glucose transporters.
UNLABELLED This study aimed to synthesize and to evaluate the biologic characteristics of (11)C labeled methyl-D-glucoside, a nonmetabolizable tracer that is selectively transported by sodium-dependent glucose transporters (SGLTs). METHODS (11)C-Methyl-D-glucoside was prepared by methylation of glucose with (11)C-methyl triflate and was obtained as a mixture of anomers that were separated wit...
متن کاملRapid, transient drop in brain glucose after intravenous phloretin or 3-0-methyl-D-glucose.
Rats were injected intravenously with either phloretin (100 mg/kg) or 3-0-methyl glucose (2 g/kg) to reduce the carrier-mediated flux of glucose into brain. Plasma glucose and brain free glucose (BFG), lactate, and glycogen were measured over a 16 min time course. Injection of these substances caused a rapid drop in BFG to 60% of control at one minute and a minimum (50% of control values) at 4 ...
متن کاملThe effect of 2-deoxy-d-glucose and 3-O-methyl-d-glucose on mucosubstance secretion in cell culture.
Ouabain at lm inhibits the secretion of hyaluronic acid by Rous-sarcoma-virustransformed hamster fibroblasts in 5.5 m-glucose medium, but not in glucose-free medium (Duguid & Marsden, 1972). As the amount of secretion in glucose-free medium is 90-95 % of that in glucose-containing medium, it appears that a complex relationship exists between mucosubstance secretion, ion transport and glucose me...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1931
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)32621-2