-Glucosidase, -Xylosidase and -L-Arabinofuranosidase Production by Mutant Trichoderma atroviride 102C1 in Different Lignocellulosic Biomass Sources
نویسندگان
چکیده
منابع مشابه
Enzymatic hydrolysis of steam-pretreated lignocellulosic materials with Trichoderma atroviride enzymes produced in-house
BACKGROUND Improvement of the process of cellulase production and development of more efficient lignocellulose-degrading enzymes are necessary in order to reduce the cost of enzymes required in the biomass-to-bioethanol process. RESULTS Lignocellulolytic enzyme complexes were produced by the mutant Trichoderma atroviride TUB F-1663 on three different steam-pretreated lignocellulosic substrate...
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A cDNA expression library of Trichoderma reesei RutC-30 was constructed in the yeast Saccharomyces cerevisiae. Two genes, abf1 and bxl1, were isolated by screening the yeast library for extracellular alpha-L-arabinofuranosidase activity with the substrate p-nitrophenyl-alpha-L-arabinofuranoside. The genes abf1 and bxl1 encode 500 and 758 amino acids, respectively, including the signal sequences...
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Trichoderma species are widely used for the commercial production of cellulolytic enzymes. In the present investigation, medium components were optimized using a central composite design and response surface methodology to produce endoglucanase (EG) from Trichoderma harzianum KUC1716. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optima...
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The carbohydrate moieties of arabinogalactan proteins (AGPs) are essential for their physiological functions and undergo rapid turnover in vivo. Degradation of the carbohydrate moieties of AGPs seems to occur by concerted action of several glycosidases, among them a-L-arabinofuranosidase, b-Dgalactosidase, and b-D-glucuronidase. Here, a bifunctional a-L-arabinofuranosidase/b-D-xylosidase from i...
متن کاملCharacterization of β-Xylosidase and α-L-Arabinofuranosidase Activities From Thermomonospora Fusca BD25
Thermomonospora fusca BD25 produces relatively high levels of activity of β-xylosidase and α-L-arabinofuranosidase. The aim of the work described in this study was to characterize some properties of β-xylosidase and α-L-arabinofuranosidase produced by T. fusca BD25 when growing on oat spelt xylan as the main carbon and energy sources. The substrates p-NPX and p-NPA reacted with β-xylosidase and...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2018
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2018.703.114