L-phenylalanine was produced by genetically modified bacterium, Escherichia coli BL21(DE3), using glycerol as an alternative carbon source. Response surface methodology (RSM) involving central composite design (CCD) was adopted to evaluate the amount of L-phenylalanine produced. In this work, the optimum concentrations were determined of the major nutrients in the fermentation medium, which inc...

Regarding some reported antimicrobial properties of tannins; Zymomonas mobilis was used to obtain ethanol from tannin-reduced carob pod extract (TR-CPE). Culture of 50 mL volume containing 7.5 g sugar at pH 5.5 and 0.03 g bacterial inoculums with shaking at 80 rpm was used. Using response surface methodology (RSM), the maximum ethanol concentration of 5.34 % w/v (higher than that reported earli...

In this study it was aimed to investigate inducibility of secondary metabolism in Astragalus chrysochlorus by yeast extract which is known to cause the synthesis of defensive phenolic metabolites. Cell suspension cultures of Astragalus chrysochlorus responded to elicitor treatment (10 gl(-1) yeast extract) by increasing phenylalanine ammonia lyase (PAL E.C. 4.3.1.5) activity the key enzyme of p...

Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi, were evaluated in two different ecosystems. Samplings taken from two areas of Atlantic forest, in Paraty-RJ, Brazil, one with a secondary forest and the other with a grass pasture were studied. Four growth media: GL (glucose and yeast extract), GES (glucose, soil extract, KNO3, CaCl2, MgSO4, NaCl, FeEDTA and micronu...

yeasts, especially saccharomyces cerevisiae, are one of the oldest organisms with broad spectrum of applications, owing to their unique genetics and physiology. yeast extract, i.e. the product of yeast cells, is extensively used as nutritional resource in bacterial culture media. the aim of this study was to develop a simple, rapid and cost benefit process to produce the yeast extract. in this ...

In this paper, we start by analyzing the presence of grey literature (GL) references in the bibliographies of different article-types using an extract of 122,406 articles from the ACM Digital Library. GL articles accounted for about 16% of the overall references with highest presence in proceedings (17.61%). Boosting techniques for promoting GL references are proposed for IR-based systems for t...

Halophilic and halotolerant microorganisms are good candidates for decolorization of azo dyeswhich are routinely used in the dyeing process in textile industries. In this paper, theoptimization of biological decolorization of synthetic dye solutions containing Remazol Black Bby the previously isolated halophilic bacterium Halomonas sp. D2 is investigated. In a primaryinvestigation using a one-f...

halophilic and halotolerant microorganisms are good candidates for decolorization of azo dyeswhich are routinely used in the dyeing process in textile industries. in this paper, theoptimization of biological decolorization of synthetic dye solutions containing remazol black bby the previously isolated halophilic bacterium halomonas sp. d2 is investigated. in a primaryinvestigation using a one-f...

BACKGROUND Efficient cofermentation of glucose and xylose is necessary for economically feasible bioethanol production from lignocellulosic biomass. Here, we demonstrate pretreatment of sugarcane bagasse (SCB) with green liquor (GL) combined with ethanol (GL-Ethanol) by adding different GL amounts. The common Saccharomyces cerevisiae (CSC) and thermophilic S. cerevisiae (TSC) strains were used ...