نتایج جستجو برای: xpert mtb/rif assay

تعداد نتایج: 222590  

Journal: :journal of research in medical sciences 0
jinghui du zikun huang qing luo guoliang xiong xiaomeng xu weiting li

background: early pleural tuberculosis (tb) diagnosis is particularly difficult. the aim of this study was to investigate the diagnostic accuracy of the xpert mtb/rif (xpert) (cepheid, sunnyvale, ca) assay using pleural biopsy and pleural fluid specimens in patients with suspected pleural tb but who had a negative sputum acid-fast bacilli (afb) smear. materials and methods: in this study, 134 s...

2013
Nakwon Kwak Sun Mi Choi Jinwoo Lee Young Sik Park Chang-Hoon Lee Sang-Min Lee Chul-Gyu Yoo Young Whan Kim Sung Koo Han Jae-Joon Yim

The Xpert MTB/RIF assay was introduced for timely and accurate detection of tuberculosis (TB). The aim of this study was to determine the diagnostic accuracy and turnaround time (TAT) of Xpert MTB/RIF assay in clinical practice in South Korea. We retrospectively reviewed the medical records of patients in whom Xpert MTB/RIF assay using sputum were requested. The sensitivity, specificity, positi...

2013
Jeong Su Park Dong-Hee Cho Jae Hyug Yang Moon Young Kim Son Moon Shin Eui-Chong Kim Sung Sup Park Moon-Woo Seong

BACKGROUND Group B streptococcus (GBS) infection is a leading cause of neonatal morbidity and mortality worldwide. Here, we present the analytical and diagnostic usefulness of a new real-time PCR-based assay (Xpert GBS; Cepheid, USA) for rapid and accurate prenatal GBS screening. METHODS We enrolled 175 pregnant women who were between 35 and 39 weeks of gestation. The analytical performance o...

Journal: :Journal of clinical microbiology 2014
Annette Blaich Reno Frei

With interest, we read the paper “Integrating the Xpert MTB/ RIF Assay into a Diagnostic Workflow for Rapid Detection of Mycobacterium tuberculosis in a Low-Prevalence Area” by Deggim et al. (1). Based on the failed detection of Mycobacterium tuberculosis complex (MTB) by the Xpert assay (GeneXpert; Cepheid, Sunnyvale, CA) in 3 of 4 culture-positive nonrespiratory specimens, the authors conclud...

2012
Saeam Shin Minkyung Kim Myungsook Kim Heejung Lim Heejung Kim Kyungwon Lee Yunsop Chong

Infection with Clostridium difficile is a growing concern because of the increasing prevalence and spread of nosocomial infections. Emergence of the hypervirulent 027/NAP1/BI strain is also notable. Existing diagnostic methods have low sensitivity or are time-consuming. Therefore, establishing a rapid and accurate microbiological diagnostic assay is needed. We evaluated the Xpert C. difficile a...

2017
Suraj Kumar Sawan Bopanna Saurabh Kedia Pratap Mouli Rajan Dhingra Rajesh Padhan Mikashmi Kohli Jigyasa Chaubey Rohini Sharma Prasenjit Das S Dattagupta Govind Makharia SK Sharma Vineet Ahuja

BACKGROUND/AIMS The use of genetic probes for the diagnosis of pulmonary tuberculosis (TB) has been well described. However, the role of these assays in the diagnosis of intestinal tuberculosis is unclear. We therefore assessed the diagnostic utility of the Xpert Mycobacterium tuberculosis/rifampicin (MTB/RIF) assay, and estimated the prevalence of multidrug-resistant (MDR) TB in the Indian pop...

Journal: :Journal of clinical microbiology 2013
Vanessa Deggim Akos Somoskovi Antje Voit Erik C Böttger Guido V Bloemberg

The Xpert MTB/RIF assay is a rapid and fully automated real-time PCR assay. The performance of the Xpert MTB/RIF assay as a primary screening test for urgent clinical specimens was evaluated during a 2-year period. The results showed that replacing smear microscopy with the Xpert MTB/RIF assay facilitates laboratory handling and improves the sensitivity and specificity of Mycobacterium tubercul...

Journal: :Journal of clinical microbiology 2010
Susan M Novak-Weekley Elizabeth M Marlowe John M Miller Joven Cumpio Jim H Nomura Paula H Vance Alice Weissfeld

The incidence of Clostridium difficile infection (CDI) has risen almost 3-fold in the United States over the past decade, emphasizing the need for rapid and accurate tests for CDI. The Cepheid Xpert C. difficile assay is an integrated, closed, nucleic acid amplification system that automates sample preparation and real-time PCR detection of the toxin B gene (tcdB). A total of 432 stool specimen...

2015
Jinghui Du Zikun Huang Qing Luo Guoliang Xiong Xiaomeng Xu Weiting Li Xu Liu Junming Li

BACKGROUND Early pleural tuberculosis (TB) diagnosis is particularly difficult. The aim of this study was to investigate the diagnostic accuracy of the Xpert MTB/RIF (Xpert) (Cepheid, Sunnyvale, CA) assay using pleural biopsy and pleural fluid specimens in patients with suspected pleural TB but who had a negative sputum acid-fast bacilli (AFB) smear. MATERIALS AND METHODS In this study, 134 s...

2014
Hee Jae Huh Byeong-Ho Jeong Kyeongman Jeon Won-Jung Koh Chang-Seok Ki Nam Yong Lee

BACKGROUND The Xpert MTB/RIF assay (Xpert assay; Cepheid, Sunnyvale, CA) is becoming the test of choice for the rapid diagnosis of tuberculosis and rifampin (RIF) resistance. The aim of this study was to evaluate the performance of the Xpert assay with respect to its clinical application at a tertiary care hospital in Korea, a country with an intermediate tuberculosis burden and high-resource. ...

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