in 2010- 2012 surveys, witches'- boom disease of tomato was observed in borazjan area (bushehr province, iran). agent of the disease was transmitted from tomato to tomato and eggplant by grafting and to madagascar periwinkle via dodder inoculation, inducing phytoplasma-type symptoms in inoculated plants. presence of phytoplasma in naturally affected tomatoes and all symptomatic graft and dodder...

DNA of 10 lines of rice yellow dwarf (RYD) mycoplasmalike organisms (MLOs) from Japan, the Phillippines, and Thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal DNA of a Tochigi (Japan) line of RYD MLO. One chromosomal probe (RYD9) and all six extrachromosomal probes hybridized with various other MLOs (sugarcane white leaf, onion yellows, ciner...

In order to explore the optimal decolorization technology of paulownia wood suitable for middle and small Enterprises to obtain its high added values, the chemical processing method was used to solve the difficult problem of paulownia wood decolorization. The results showed that: 1) temperature, pressure, time and treatment solution concentration all had significant effects on the decolorizatio...

DNA was isolated from clover proliferation (CP) mycoplasmalike organism (MLO)-diseased periwinkle plants (Catharanthus roseus (L.) G. Don.) and cloned into pSP6 plasmid vectors. CP MLO-specific recombinant DNA clones were biotin labeled and used as probes in dot hybridization and restriction fragment length polymorphism analyses to study the genetic interrelatedness among CP MLO and other MLOs,...

MicroRNAs (miRNAs) play an important role in responding to biotic and abiotic stresses in plants. Jujube witches'-broom a phytoplasma disease of Ziziphus jujuba is prevalent in China and is a serious problem to the industry. However, the molecular mechanism of the disease is poorly understood. In this study, genome-wide identification and analysis of microRNAs in response to witches'-broom was ...

Hibiscus rosa-sinensis is a valuable ornamental species widely planted in Brazil. Many plants are affected by witches' broom disease, which is characterized by excessive axillary branching, abnormally small leaves, and deformed flowers, symptoms that are characteristic of diseases attributed to phytoplasmas. A phytoplasma was detected in diseased Hibiscus by amplification of rRNA operon sequenc...

The trivial name 'phytoplasma' has been adopted to collectively name wall-less, non-helical prokaryotes that colonize plant phloem and insects, which were formerly known as mycoplasma-like organisms. Although phytoplasmas have not yet been cultivated in vitro, phylogenetic analyses based on various conserved genes have shown that they represent a distinct, monophyletic clade within the class Mo...

the witches’ broom disease of lime trees is a destructing disease caused by the bacterium candidatus phytoplasma aurantofolia (1). the disease is prevalent in oman,united arab emirates and iran. to restrict the spread ofthe infected trees, detection of the causative bacteriumis crucial. besides pcr-based methods, antibodies can beapplied for detection of pathogen-infected cells.recently shahrya...

Phytoplasma is an insect-transmitted pathogen that causes witches' broom disease in many plants. Paulownia witches' broom is one of the most destructive diseases threatening Paulownia production. The molecular mechanisms associated with this disease have been investigated by transcriptome sequencing, but changes in protein abundance have not been investigated with isobaric tags for relative and...