نتایج جستجو برای: upstream and downstream fragments from target gene and antibiotic resistance cassette from plasmid pet28a ( )

تعداد نتایج: 17827133  

Journal: :تحقیقات دامپزشکی 0
سولماز ناصرلی گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه تهران تقی زهرایی صالحی گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه تهران بهار نیری فسایی گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه تهران علیرضا سعیدی نیا گروه فناوری زیستی، دانشگاه صنعتی مالک اشتر ایرج اشرافی تمامی گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه تهران

background: causing site direct mutation can be one of the efficient methods to evaluate the characteristics and properties of various genes. brucellosis is the most common zoonotic infectious disease that would cause great economic losses. thus, recognition of pathogenic and immunogenic factors in the genus brucella can lead to control this health problem. objectives: considering the importanc...

Introduction: Salmonellosis is an infection caused by eating contaminated food with Salmonella, and it can occur in humans and other animals. Salmonella has acquired the ability to create the infection due to the presence of several virulence genes. One of the virulence genes of salmonella is sipC gene that coding the SipC protein. The aim of this study was creating the gene cassette to genetic...

Journal: :Applied and environmental microbiology 2015
Man Hwan Oh Je Chul Lee Jungmin Kim Chul Hee Choi Kyudong Han

The traditional markerless gene deletion technique based on overlap extension PCR has been used for generating gene deletions in multidrug-resistant Acinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of the PCR products in DNA cloning and the construction of new vectors containing a suitable antibiotic resistance cassette for the selection o...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شاهد - دانشکده علوم پایه 1388

introduction acetic acid bacteria are large group of obligate aerobic gram negative bacteria with the ability to oxidize ethanol to acetic acid (1). they are widely distributed in natural habitats and classified in family acetobacteraceae. members of this family are useful in industrial production of vinegar(2). acetic acid bacteria (aab) can use substrates as glucose, ethanol, lactate or glyc...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده ادبیات و زبانهای خارجی 1390

august wilson is undoubtedly one of the rare black playwrights whose works have attracted streams of attention and worldwide audience. the present study aims at analyzing fences and piano lesson which are two of the most successful plays in his pittsburgh cycle from the perspective of michel foucault’s theories and ideas. studying these two plays from foucault’s perspective opens new windows in...

پایان نامه :0 1374

the research questions were as follows: 1. is there any relationship between the students concious awareness of the form and implications of the conjuncations and their improvement in using appropriate conjunctions? 2. does students knowledge of the from and the implications of the conjunctions help them to produce more coherent writings. 3. does a comparison between english conjunctions and th...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سیستان و بلوچستان - دانشکده مهندسی عمران 1391

in this study, scour of mashkid bridge pier located in mashkid river of saravan county has been studied and to measure the scour rate, the numerical model hec-ras 4.0 has been used. upon selection of mashkid river as the case study and whereas the studied zone is important with respect to the climatic conditions and showery monsoon raining and flowing the great and destructive floodwaters, ther...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علامه طباطبایی - دانشکده ادبیات و زبانهای خارجی 1387

the present study is an experimental case study which investigates the impacts, if any, of skopos on syntactic features of the target text. two test groups each consisting of 10 ma students translated a set of sentences selected from advertising texts in the operative and informative mode. the resulting target texts were then statistically analyzed in terms of the number of words, phrases, si...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده زبانهای خارجی 1392

the extent to which written corrective feedback on linguistic errors can play a role in helping l2 writers improve the accuracy of their writing continues to be an issue of interest to researchers and teachers since truscott (1996) mounted a case for its abolition. while there is growing empirical evidence that written corrective feedback can successfully target some types of linguistic error (...

Esmaeili , Aghil, Ghofrani, Mahdi, Honari, Hossein, Safaei, Sadegh,

Background & Objective: In order to enhance the expression of soluble proteins and facilitate their purification and development of multi-functional polypeptide , chimerical recombinant proteins have been invented . The purpose of this study was to construct ctxB-gfp-stxB gene cassette to measure the uptake and excretion of chimerical antigen in future studies.   Materials & Methods: After prep...

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