background: the well documented source for adult multipotent stem cells is spermatogonial stem cells (sscs). they are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. the aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type a spermatogonia in diss...

Background:Spermatogenesis is maintained by a pool of the spermatogonia. The group formed by As, Apr and Aal is known as undifferentiated type a spermatogonia (Aund). The Aal spermatogonia will differentiate, with no mitotic division, to the differentiating type A1 spermatogonia. The type A1 spermatogonia undergo 6 successive mitotic divisions to yield A2, A3, A4, intermediate-type (In), and ty...

The existence of two classes of spermatogonial stem cells in the rat testis, i.e., reserve type A0 spermatogonia and renewing, types A1-A4 spermatogonia, postulated by Clermont and Bustos-Obregon ('68), was reexamined in a quantitative analysis of type A spermatogonia in both whole mounts of tubules and in radioautographed sections of testes from animals killed at various times, up to 26 days, ...

A procedure is described which permits the isolation from the prepuberal mouse testis of highly purified populations of primitive type A spermatogonia, type A spermatogonia, type B spermatogonia, preleptotene primary spermatocytes, leptotene and zygotene primary spermatocytes, pachytene primary spermatocytes and Sertoli cells. The successful isolation of these prepuberal cell types was accompli...

The spermatogenesis and oogenesis-specific transcription factor Sohlh2 is normally expressed only in premeiotic germ cells. In this study, Sohlh2 and several other germ cell transcripts were found to be induced in mouse embryonic stem cells when cultured on a feeder cell line that overexpresses bone morphogenetic protein 4. To study the function of Sohlh2 in germ cells, we generated mice harbor...

The field of spermatogonial stem cell (SSC) technologies provides tools for genetic improvement of cattle herds and multiple opportunities for research. Spermatogonial stems cells belong to the male germ line and as such have high developmental potential, which offers many possibilities for transfer of relevant genetic traits across herds in a timely manner. Type A spermatogonia include a very ...

Unlike mammals, there is little fundamental information about spermatogenesis in birds. This study was undertaken to clarify the morphology, histochemistry, and lectin affinity of the seminiferous epithelial cells and Leydig cells in pre-pubertal (8- to 15-week old) and adult (40- to 44-week old) domestic turkeys. In adult turkeys, three types of spermatogonia were defined based on their chroma...

For the purpose of clarifying the histopathological effects of methotrexate (MTX) on medaka testes, wild-type and homogenic p53-deficient male medaka at 4 to 6 months post-hatching were exposed to 0.25 mg/ml of MTX for 96 h with histopathological examination of testes at 24, 48, 72 and 96 h. At 72 and 96 h after the start of MTX exposure, numerous apoptotic cells were observed in the spermatogo...

Spermatogonial stem cells are the only stem cells in the body that transmit genetic information to offspring. Although growth factors responsible for self-renewal of these cells are known, the factors and mechanisms that attract and physically maintain these cells within their microenvironment are poorly understood. Mice with targeted disruption of Ets variant gene 5 (Etv5) show total loss of s...

background: the present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. materials and methods: sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. isolated spermatogonial cells were co-cultured with sertoli cells in minimal essential medi...