نتایج جستجو برای: two-promoter vector
تعداد نتایج: 2651774 فیلتر نتایج به سال:
there are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. bicistronic system has disadvantages e.g. compared with two-promoter system. in this paper, a simple method based on spliced overlap extension by polymerase chain reaction (soe-pcr) technique was demonstrat...
There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrat...
in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...
the interaction between immune cells and endothelial lining of blood vessels is vital in many processes such as inflammatory and immune responses as well as cancer cell metastasis. the expression level of vcam-1 is regulated by many factors including the promoter activity that is possibly affected by the single nucleotide polymorphisms (snps) present in the promoter. there are previous reports ...
target tracking is the tracking of an object in an image sequence. target tracking in image sequence consists of two different parts: 1- moving target detection 2- tracking of moving target. in some of the tracking algorithms these two parts are combined as a single algorithm. the main goal in this thesis is to provide a new framework for effective tracking of different kinds of moving target...
Background: The micro-ribonucleic acids (miRNAs) are noncoding RNA molecules that are conserved developmentally and include usually 18-25 nucleotides. MiRNA regulates gene expression through mRNA degradation or inhibiting of its translation. These biomolecules contribute in cellular physiologic and pathologic processes and most of them may act as oncogenes or tumor inhibitors. Identification of...
OF2 and VAP genes, probably involved in signal transduction of sugarbeet nematode resistance, have already been cloned in bacterial vector by AFLP molecular marker and a two-hybrid system, respectively. To examine their capability to introduce resistance in sugarbeet, the genes were transferred to plant expression vectors. For this reason, OF2 gene after isolation was inserted within T-DNA of p...
OF2 and VAP genes, probably involved in signal transduction of sugarbeet nematode resistance, have already been cloned in bacterial vector by AFLP molecular marker and a two-hybrid system, respectively. To examine their capability to introduce resistance in sugarbeet, the genes were transferred to plant expression vectors. For this reason, OF2 gene after isolation was inserted within T-DNA of p...
objective: in an attempt to develop safer and more effective gene therapy approaches as a realistic treatment for various forms of cancer, researchers are increasingly using tumor-specific promoters (tsp) to drive the expression of the gene of interest and eradicate cancer cells. in this study, for the first time we introduce the oct-4 promoter as a cancer-specific promoter with a high efficacy...
background: the transcription factor oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. establishment of an oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro. methods: in the present study, we report construction of a recombin...
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