BACKGROUND Scorpion venom glands produce some antimicrobial peptides (AMP) that can rapidly kill a broad range of microbes and have additional activities that impact on the quality and effectiveness of innate responses and inflammation. OBJECTIVES In this study, we reported the identification of a cDNA sequence encoding cysteine-free antimicrobial peptides isolated from venomous glands of thi...

background: the mesobuthus eupeus scorpion venoms are known to contain α-toxin peptides, many of which interfere with k+ ion channel function. these toxin peptides have important value in  the pharmacology and physiology studies of specific k+ channel of cells. objectives: given the  lack of information of the molecular biology of peptides in the toxin of iranian scorpions, the aim of this stud...

objectives in this study, we reported the identification of a cdna sequence encoding cysteine-free antimicrobial peptides isolated from venomous glands of this species. materials and methods total rna was extracted from the iranian mesobuthus eupeus venom glands, and cdna was synthesized by using the modified oligo (dt). the cdna was used as the template for applying semi-nested rt- pcr techniq...

Scorpions are generally an important source of bioactive components, including toxins and other small peptides as attractive molecules for new drug development. Mesobuthus eupeus, from medically important and widely distributed Buthidae family, is the most abundant species in Iran. Researchers are interesting on the gland of this scorpion due to the complexity of its venom. Here, we have analyz...

The venoms of Buthidae scorpions are known to contain basic, single-chain protein -toxins consisting of 60-70 amino acid residues that are tightly cross-linked by four disulfide bridges. Total RNA was extracted from the venom glands of scorpion Mesobuthus eupeus collected from the Khuzestan province of Iran and then cDNA was synthesized with the modified oligo (dT) primer and extracted total R...

Scorpions are generally an important source of bioactive components, including toxins and other small peptides as attractive molecules for new drug development. Mesobuthus eupeus, from medically important and widely distributed Buthidae family, is the most abundant species in Iran. Researchers are interesting on the gland of this scorpion due to the complexity of its venom. Here, we have analyz...

In this study, Mesobuthus gibbosus and Mesobuthus eupeus eupeus venom samples were compared for lethality, in-vivo effects and proteins. Neutralization capacity of monovalent Androctonus crassicauda antivenom (RSHA anti-Ac) was tested against the lethal effects of the venoms. Venom was obtained from mature scorpions by electrical stimulation of the telson. The lethality of the venom and potency...

This work aimed to study the characterization and isolation of a alpha neurotoxinBmK, from the venom glands of Scorpion Buthida Mesobuthus eupeus Kuzestan (Iran). Scorpions Mesobuthus eupeus were collected from the Khuzestan province. Using RNXTM solution, the total RNA was extracted from the twenty separated venom glands. cDNA was synthesized with extracted total RNA as template and modified o...

Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purifi...

introduction: iranian scorpion species are classified in buthidae and scorpionidae with 16 genera and 25 species. in iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming. mesobuthus eupeus is the most common species in iran. its venom contains several toxin fractions which can affect the ion channel. in this study purification, ...