ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for ...

The ITS region including the 5.8S gene of rDNA of three desert truffle species were amplified using ITS4 and ITS1 primers. The ITS sequences were compared to those of other related authentic sequences obtained from GenBank. Among 12 specimens studied, seven isolates corresponded to Terfezia claveryi reported by other authors. Iranian T. claveryi specimens had an average similarity of 99.4% (ran...

abstract- desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. a pcr-based method was developed for the identification of 3 major desert truffles of iran: terfezia claveryi, tirmania pinoyi and tirmania nivea based on internal transcribed spacers of rdna. two specific pcr primers were designed for t. claveryi, 4 for t. pinoyi, and 2 for ...

fungi associated with ascocarps of terfezia claveryi, tirmania nivea, t. pinoyi, picoa lefebvrei and p. juniperi in iran showed a wide range of genera and species in 94 samples collected during 2005–2009 from different regions including fars, systan and baluchestan, kerman, east azarbaijan, khuzestan, kermanshah and hormozgan provinces. thirty two species belonging to 21 genera were recovered f...

terfezia, picoa and tirmania, so called desert truffles, are mycorrhizal fungi mostly endemic to arid and semi-arid areas of the mediterranean region, where they are associated with helianthemum and carexspecies. the aim of this work was to study the identification, molecular analysis, distribution and hosts of these pezizalean hypogeous fungi in iran. among the specimens, terfezia claveryi, ti...