نتایج جستجو برای: syber green real time PCR

تعداد نتایج: 2384944  

Journal: :journal of dental materials and techniques 0
naser sargolzaie dental research center, department of periodontology, mashhad university of medical sciences, mashhad, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (mashhad university of medical sciences) saeed amel-jamedar department of microbiology, ghaem educational, research, and treatment center, mashhad university of medical sciences, mashhad, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (mashhad university of medical sciences) majid reza mokhtari 3 oral and maxillofacial diseases research center, school of dentistry, mashhad university of medical sciences, mashhad, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (mashhad university of medical sciences) hamid reza arab dental research center, department of periodontology, mashhad university of medical sciences, mashhad, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (mashhad university of medical sciences) soodabe piroozi general dentist, mashhad, iran

introduction: one of the most common problems we confront in orthodontic therapy is periodontal diseases. initial factor which causes these diseases is colonization of anaerobic microorganisms in subgingival plaque. technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. however, it seems necessary to assess va...

Journal: :The new microbiologica 2010
Domenica Donia Anna Rita Petrinca Maurizio Divizia

Various methods for the recovery and detection of HAV have been suggested, and molecular tests have recently provided an effective replacement for the traditional methods. Real-time RT-PCR technology offers many advantages over conventional RT-PCR in terms of rapidity and specificity. Most procedures are based on the TaqMan chemistry, but some researchers have used the SYBR Green I approach, wh...

ژورنال: :مجله دانشکده پزشکی اصفهان 0
ثریا احمدی بلوطکی گروه ژنتیک، دانشکده ی علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران مجتبی عمادی بایگی استادیار، گروه ژنتیک، دانشکده ی علوم پایه و پژوهشکده ی بیوتکنولوژی، دانشگاه شهرکرد، شهرکرد، ایران لیلا روحی استادیار، گروه زیست شناسی تکوین جانوری، دانشکده ی علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران علی اکبر شایسته استادیار، گروه داخلی، دانشکده ی پزشکی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران

مقدمه: سرطان مری، ششمین سرطان منجر به مرگ در دنیا می باشد. اگر چه بروز این بیماری در سه دهه ی گذشته در ایران رو به کاهش بوده است، اما همچنان ششمین سرطان شایع در کشور می باشد. ژن tox3 به خانواده ای بزرگ و متنوع از پروتئین های hmg-box تعلق دارد که روی کروموزوم 16q12 قرار گرفته است. مطالعه ی حاضر با هدف مقایسه ی بیان ژن tox3 در بافت پارافینه ی سرطانی و غیر توموری مری انجام شد. روش ها: این مطالعه ،...

Journal: :Pathobiology : journal of immunopathology, molecular and cellular biology 2009
Jin-Heng Xu Yu-Chuan Wang Xin Geng Yan-Yun Li Wei-Ming Zhang

OBJECTIVE We attempted to reveal the changes of the human telomerase reverse transcriptase (hTERT) alternative splicing pattern in gastric carcinogenesis. METHODS Three alternative splicing sites (alpha, beta, gamma) were selected and designed PCR primer. The expression of 8 hTERT alternative splicing variants (ASVs) in normal gastric mucosa, precancerous lesions and gastric cancer were detec...

Journal: :avicenna journal of medical biotechnology 0

background: asthma is caused by the combination of different factors. current concepts of asthma pathogenesis emphasize on gene-environment interactions. mega-genome scanning projects revealed that different single nucleotide polymorphisms (snps) are related to asthma susceptibility. rs7216389-t is one of them that is related to childhood asthma and its effect on childhood asthma severity has b...

Journal: :jundishapur journal of microbiology 0
mozhdeh sultani virology department, school of public health, tehran university of medical sciences, tehran, ir iran talat mokhtari azad virology department, school of public health, tehran university of medical sciences, tehran, ir iran mohammadreza eshragian virology department, school of public health, tehran university of medical sciences, tehran, ir iran azadeh shadab virology department, school of public health, tehran university of medical sciences, tehran, ir iran maryam naseri virology department, school of public health, tehran university of medical sciences, tehran, ir iran owrang eilami department of infectious disease, yasuj university of medical sciences, yasuj, ir iran

conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...

ژورنال: دانشور پزشکی 2018
اولیاء, پرویز, دهقان زاده, زهرا, صادری, حوریه, مرعشی, سید محمود امین, مکاری, مریم,

Background and Objective: Saccharomyces cerevisiae is one of the probiotic yeasts that has positive effects on health. Pseudomonas aeruginosa is one of the important opportunistic pathogenic bacteria. The purpose of this study was to determine the effect of supernatant of Saccharomyces cerevisiae on preventing the growth of Pseudomonas aeruginosa and its effect on exotoxin S gene expression by ...

Journal: :Nucleic acids research 2003
R G Rutledge C Côté

Fluorescent monitoring of DNA amplification is the basis of real-time PCR, from which target DNA concentration can be determined from the fractional cycle at which a threshold amount of amplicon DNA is produced. Absolute quantification can be achieved using a standard curve constructed by amplifying known amounts of target DNA. In this study, the mathematics of quantitative PCR are examined in ...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
saeid solali department of hematology, faculty of medical science, tarbiat modares university, tehran, iran saeid kaviani assistant professor, department of hematology, faculty of medical sciences, tarbiat modares university, tehran, iran p.o.code: 1411713116 ali akbar movassaghpuor assistant professor, hematology and oncology research center, tabriz university of medical science, tabriz, iran mohammad reza ali-parasti department of immunology, faculty of medical science, tabriz university of medical science, tabriz, iran masoud soleimani assistant professor, department of hematology, faculty of medical sciences, tarbiat modares university, tehran, iran mehrdad noruzinia assistant professor, department of hematology, faculty of medical sciences, tarbiat modares university, tehran, iran saeid abroun

هدف: در این مطالعه بیان ژن‏های mdr1 و hoct1 در بیماران مبتلا به لوسمی میلوئیدی مزمن و افراد طبیعی با استفاده از rt-real time pcr مطالعه شد. مواد و روش‏ها: برای ارزیابی بیان ژن از سیستم real-time pcr با syber green master-mix استفاده شد. از تعداد 30 بیمار مبتلا به لوسمی میلوئیدی مزمن و 27 فرد سالم نمونه‏گیری شد. نتایج real time-pcr به روش سنجش نسبی ارزیابی شد. نتایج: نتایج تحقیق حاضر نشان داد که...

Journal: :modares journal of medical sciences: pathobiology 2011
saeid solali saeid kaviani ali akbar movassaghpuor mohammad reza ali-parasti masoud soleimani

objective: in this study quantitative expression of mdr1 and hoct1 genes in cml patients and normal people were measured using real-time pcr. materials and methods: to study quantitative expression of these genes by real-time pcr, master-mix with syber green was used. peripheral blood samples from 30 cml patients and 27 normal persons were harvested. real-time pcr results were analyzed with re...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید