the aim pf the present study was to compare three different methods for detection of food and mouth disease virus antigens(a, asia1, o1)from mouth epithelium samples and also to monitore the distribution of fmdv serotypes 338 cattle and 34 sheep of iran during 2000-2001. the suspension fluids of processed tongue epithelium samples were clarified by centrifugation and tested for presence of fmdv...

Int J Vaccines Vaccin 2016, 3(2): 00060 Abbreviations: FMDV: Foot and Mouth Disease Virus; BHK: Baby Hamster Kidney; CPE: Cytopathic Effects; ELISA: Enzyme Linked Immunosorbent Assay; FMD: Foot and Mouth Disease; CFT: Complement Fixation Test, VNT: Virus Neutralization Test; PBS: Phosphate Buffered Saline; IF: Infectious Fluids; OD: Optical Density; BA: Bacteriological Media; ELISA: Enzyme Link...

Pasteurella multocida (P. multocida) is an important pathogen of various domestic animals. The outer membrane proteins (OMPs) play a major role in pathogenesis and immunogenicity of P. multocida. The aim of the study was to develop indirect enzyme linked immuno sorbant assay (ELISA) based on OMPs to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. The ...

A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID(50)) test (TCID(50)-ELISA) was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by TCID(50)-ELISA were comparable to those obtained by the plaque assay and by the traditional TCID(50)-cytopathic e...

For establishment of plaque assay on BHK-21(Cl-13) cell line, different parameters were standardized. A cell count of 3-4 X 10 5 /ml was found suitable when grown in Eagle’s minimum essential medium (EMEM) containing 5% foetal bovine serum (FBS) whereas a concentration of 2% FBS was optimum for the overlay media containing 0.2% final conc. of carboxymethyl cellulose (CMC); a pH value of 7. 8 wa...

Pasteurella multocida (P. multocida) is an important pathogen of various domestic animals. The outer membrane proteins (OMPs) play a major role in pathogenesis and immunogenicity of P. multocida. The aim of the study was to develop indirect enzyme linked immuno sorbant assay (ELISA) based on OMPs to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. The ...

A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 we...

pasteurella multocida (p. multocida) is an important pathogen of various domestic animals. the outer membrane proteins (omps) play a major role in pathogenesis and immunogenicity of p. multocida. the aim of the study was to develop indirect enzyme linked immuno sorbant assay (elisa) based on omps to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. the ...

Following an outbreak of fascioliasis in Anzali Harbor in 1988, 452 blood samples were collected using a multistage sampling method for hematological and serological studies. After determination of eosinophilia in the samples, antifasciolia antibodies in the sera were also determined using enzyme linked immuno sorbant assay (ELISA) and counter-current immuno electrophoresis (CCIE). 64.2% ...

Hepatitis B and hepatitis C are among the most significant hepatic infections all around the world that may lead to hepatocellular carcinoma. This study is first time performed at the blood transfussion centre of Omar hospital, Lahore. It aims to determine the sero-prevalence of these diseases by screening the apparently healthy blood donors who might be the carriers of HBV or HCV and pose a hi...