Calf-thymus nucleohistone studied by a newly developed ;SDS gradient' centrifugation technique showed that histones dissociate sequentially when treated with increasing concentrations of sodium dodecylsulfate. Histones F2a1 and F2a2 were dissociated first at about 0.03% sodium dodecylsulfate, and F1 was removed lastly by the highest concentration of sodium dodecylsulfate (0.06% or more). A DNA-...

Electrospray ionization/tandem mass spectrometry of proteins separated on sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels is severely limited by the requirement that the protein be completely separated from the SDS. As shown here, the gaseous noncovalent SDS adducts of protonated proteins thus formed can be dissociated by infrared irradiation. ESI spectra from myoglobin...

Abstract The impregnation of granular activated carbon with the anionic surfactant sodium dodecylsulfate (SDS) was studied and influence on aurocyanide adsorption evaluated. Thermodynamic kinetic parameters were also determined. SDS improves gold by 10%, showing a cleansing effect surface which displayed in higher specific area. Specific pore volume average diameter did not undergo important ch...

a newly developed natural rubber deproteinization process produces deproteinized natural rubber (dpnr) wastewater as an intermediate product containing a high concentration of sodium dodecyl sulfate (sds) and rubber. in this study, a novel process to recover the residual rubber and energy as methane from dpnr wastewater was developed. as a pretreatment, sds and residual rubber in dpnr wastewate...

Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...

A running colour electrophoresis of protein in sodium dodecylsulfate (SDS)-polyacrylamide gel (PAG) has been developed and the coloured gel was restained with silver nitrate and hydroquinone (HQ) within 20min. Trichloroacetic acid (TCA) was found to be a powerful destaining reagent and reproducibility of this double staining procedure was excellent.

Anionic surfactant solutions of sodium dodecylbenzenesulfonate (SDBS) and sodium dodecylsulfate (SDS) separate into two phases through cooling of the solution and the addition of salts. Thiourea (TU) reacts with many metals to form cationic complexes of [M(TU)(n)](m+); some of them extract into the SDBS phase. The separation was examined for lead and tin in commercially purchased solders. More ...

We report on enhanced experimental optical activity measurements of thin soap films in the presence of sugar. This unusual optical activity is linked to the intramolecular chiral conformation of the glucose molecules at the air/liquid interface. Choosing sodium dodecylsulfate (SDS) as a model surfactant and glucose as model sugar, favorable interactions between the anionic group -OSO3(-)- and t...

Patterns of assembled colloidal particles can form on substrates due to solvent evaporation, and here we studied such phenomena in the drying monodispersed hydrochar dispersions prepared by hydrothermal carbonization glucose purified dialysis. During evaporation water, line patterns or, some cases, mud-like formed. The formation was investigated as a function pH dispersion, substrate shape, par...