conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

we have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-de). this modified version of 2-de is not only simpler and easier but also faster than all the currently available methods. in this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-...

This study investigated calcium sulfate and chitosan on the textural modification microstructure of tofu made from lentils. The addition varying amounts (0–12 mM) (0–1.0%) into lentil milk could affect gel properties lentil-based tofu. properties, including hardness cohesiveness, significantly increased with 12 mM sulfate, exhibiting a slightly discontinuous network structure regular pore netwo...

Renin was purified from 47 kg of hog kidney to produce enough enzyme for enzymatic and physicochemical characterization. The procedure included extraction at pH 3.5 in the presence of protease inhibitors, two ammonium sulfate precipitations, ion exchange chromatography on Sepharose-hexamethylenediamino-pepstatin gel, gel filtration, and isoelectric focusing. Renin, 2.3 mg, with a specific activ...

1 The abbreviations used are: KDO, 3-Deoxy-D-manno-octulosonate; KDO 8-P, 3-DeoxyD-manno-octulosonate 8-phosphate; Tris, Tris-(hydroxymethyl)aminomethane; HEPES, N-[2Hydroxyethyl]piperazine-N’-[2-ethanesulfonic acid]; PNPase, purine nucleoside phosphorylase; EDTA, (ethylenedinitrilo)tetraacetic acid; 2D gel electrophoresis, two dimensional gel electrophoresis; SDS-PAGE, sodium dodecyl sulfate-p...

Volume 1 • Issue 7 • 1000e125 Biochem Anal Biochem ISSN:2161-1009 Biochem, an open access journal Electrophoresis is one of the research techniques, which has extensively been used in separation and identification of proteins/ enzymes. In majority of the biochemistry and molecular biology experiments, electrophoresis of proteins and enzymes is performed using polyacrylamide gel electrophoresis ...

Abstract In this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These were used to extract residual proteins in non-aged and thermal aged samples. The protein efficiencies different extracting quantitatively determined by bicinchoninic acid (BCA) method, then processed multivariate analysis variance (MANOVA). impact pr...

Sodium dodecyl sulfate (SDS) is a strong surfactant that is widely used in protein sample preparation. While protein and peptide samples containing up to approximately 1% SDS can be analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) using a two-layer matrix/sample deposition method, the presence of SDS in a protein sample generally degrades mass resolution and ...

Abbreviations : apo, apolipoprotein; CE-OOH, cholesteryl ester hydroperoxides; RP-HPLC, reversed-phase high-performance liquid chromatography; LDL, low-density lipoprotein; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TBARS, 2-thiobarbituric acid-reactive substances; TBS, Tris-HCl-buŠered saline; a-Toc, a-tocopherol; VLDL, very-low-density lipoprotein Biosci. Biotechnol....