serology and dna techniques are employed for human leukocyte antigen (hla) typing in different transplant centers. results may not always correlate well and may need retyping with different technique. all the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring hla typing for bone marrow transplant were enrolled in the study. serological hla typing was ...

The present study aimed to explore the factors that affect polymerase chain reaction using sequence-specific primers (PCR-SSP) and to establish an optimized PCR-SSP method for detecting multiple gene polymorphisms simultaneously. The amplification system parameters, including the concentrations of Mg2+, dNTPs, pfu Taq, primers and control primers, were optimized using the designed PCR-SSP react...

barley belongs to the poaceae which is the largest monocotyledon family. plastid single-copy gene acetyl-coa carboxylase (accase) is the first step in the biosynthesis of fatty acids and therefore, it is used to study the phylogenetic relationships, evolutionary and systematic of grasses. in this study, for the first time, phylogenetic relationship of eight species of hordeum genus from iran in...

PCR using sequence-specific primers (PCR-SSP) is widely employed for the genotyping of single nucleotide polymorphisms (SNPs) in both routine diagnosis and medical research. The human platelet alloantigens (HPAs) represent SNPs in platelet-specific glycoproteins, and HPA-1, -2, -3, and -5 are the most relevant in immunohematology. In most protocols, the respective HPA-SNPs are analyzed in allel...

and Granulocyte Immunobiology, S’Agaró, Girona, Spain, May 9-12, 1998, p 52 (abstr) 6. Hessner MJ, Curtis BR, Endean DJ, Aster RH: Determination of neutrophil antigen NA gene frequencies in five different ethnic groups by the polymerase chain reaction with sequence-specific primers (PCR-SSP). Transfusion 36:895, 1996 7. Lin M, Chen CC, Wang CL, Lee HL: Frequencies of neutrophilspecific antigens...

and Granulocyte Immunobiology, S’Agaró, Girona, Spain, May 9-12, 1998, p 52 (abstr) 6. Hessner MJ, Curtis BR, Endean DJ, Aster RH: Determination of neutrophil antigen NA gene frequencies in five different ethnic groups by the polymerase chain reaction with sequence-specific primers (PCR-SSP). Transfusion 36:895, 1996 7. Lin M, Chen CC, Wang CL, Lee HL: Frequencies of neutrophilspecific antigens...

and Granulocyte Immunobiology, S’Agaró, Girona, Spain, May 9-12, 1998, p 52 (abstr) 6. Hessner MJ, Curtis BR, Endean DJ, Aster RH: Determination of neutrophil antigen NA gene frequencies in five different ethnic groups by the polymerase chain reaction with sequence-specific primers (PCR-SSP). Transfusion 36:895, 1996 7. Lin M, Chen CC, Wang CL, Lee HL: Frequencies of neutrophilspecific antigens...