syntax of OWL LITE139 Instances of Protocol: PCRSupermixProtocol : Protocol HighMWRunningBuffer5xProtocol : BufferProtocol LowMWRunningBuffer5xProtocol : BufferProtocol TAEBufferProtocol : BufferProtocol GelBuffer3P5XProtocol : BufferProtocol AffymetrixDNALabellingForGeneExpressionArrays : InV itroProtocol AgaroseGelElectrophoresis : InV itroProtocol SDSPAGE : InV itroProtocol BacterialCell...

Riboflavin-binding protein (RfBP) was isolated, purified and characterized from Coot (Fulica atra) egg. The RfBP was purified using DEAE-Sepharose ion exchange chromatography followed by gel filtration on Sephadex G-100. The purity of the proteins was judged by SDSPAGE. The protein migrated as a single band on SDS gel with a molecular weight of corresponding to nearly 29 Kd.

Gloeothece is a non-heterocystous, unicellular cyanobacterium that is capable of aerobic N, fixation [I]. As in other diazotrophs, nitrogenase in Gloeothece consists of a MoFe-protein and an Feprotein. The Faprotein of Gloeothece nitrogenase can be resolved into two different, though antigenically similar, forms by SDSPAGE. These two forms have apparent M, values of 38 500 and 40 000 respective...

Sericin is a hot water soluble glycoprotein and has been partially characterized in the domesticated silkworm Bombyx mori. Contrary to this very little information is available about the sericin of non mulberry silkworms. Sericin is generally a waste product during degumming. Recent advancement in silkworm research has revealed the use of sericin of B. mori as protein based biomaterial involvin...

Using gas–mass chromatography analyses (GS-MS) we have determined the endogenous levels of jasmonic acid (JA) in developing sunflower seeds. The level of JA was higher in the early phases of seed development. We have registered a maximum on day 5 after pollination. Spraying of plants with JA at the time of flowering induced marked changes in protein content. Five days after treatment total prot...

An extracellular protease-producing actinomycete, Streptomyces nogalator strain Ac 80, was isolated from soil. Mutants of the strain designated as Mt C producing protease of higher (3.0 mg tyrosine liberated/min/mg protein) and Mt L of lower (0.9 mg tyrosine liberated/min/mg protein) specific activity as compared to that of the wild type (1.8 mg tyrosine liberated/min/mg protein) were selected....

Riboflavin binding protein was purified from the egg yolk of Aquila hastate (Eagle). The protein was purified using DEAE Sepharose ion exchange chromatography followed by gel filtration on Sephadex G-100. The purity of the protein was judged by cylindrical and slab SDSPAGE techniques. Comparison of the mobility of RfBP with that of the standard molecular weight marker protein revealed that the ...

Methods A phage library of 7000 clones was constructed from a drug naive HIV-1 clade C infected Indian patient whose plasma exhibited high potential neutralizing potential against a panel of viruses and also displayed cross-reactive anti-V3 antibodies. PBMCs were isolated and EBV transformed. Cells (wells) producing anti-V3 antibodies were preselected with V3-CTB fusion protein and expanded. To...

:Laccases are oxidoreductases enzymes that belong to the sub class copper oxidases.They mainly produced by white rot fungi which involved in lignin metabolism. The objectiveof this research was effective utilization of agro wastes for economical production laccasefrom, Pleurotusflorida. Submerged fermentation employed enzymeand namely rice husk, wheat bran and sugarcane bagasse used as substrat...

Lipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available concerning LPS physiology in different symbiotic (parasitic) and pathogenic host-bacterial inter...