The genetic structure of a population of nonsymbiotic Rhizobium leguminosarum strains was determined by the electrophoretic mobilities of eight metabolic enzymes. Nonsymbiotic strains were isolated from the rhizosphere of bean plants and characterized by growth on differential media and at different temperatures, intrinsic antibiotic resistance, the lack of homology to a nifH probe, and their i...

Abstract A collection of 160 isolates of rhizobia nodulating Phaseolus vulgaris in three geographical regions in Tunisia was characterized by restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified 16S rDNA, nifH and nodC genes. Nine groups of rhizobia were delineated: Rhizobium gallicum biovar (bv.) gallicum, Rhizobium leguminosarum bv. phaseoli and bv. v...

BacA is an integral membrane protein, the mutation of which leads to increased resistance to the antimicrobial peptides bleomycin and Bac7(1-35) and a greater sensitivity to SDS and vancomycin in Rhizobium leguminosarum bv. viciae, R. leguminosarum bv. phaseoli, and Rhizobium etli. The growth of Rhizobium strains on dicarboxylates as a sole carbon source was impaired in bacA mutants but was ove...

Many bacterial species exhibit strong linkage disequilibrium of their chromosomal genes, which apparently indicates restricted recombination between alleles at different loci. The extent to which restricted recombination reflects limited migration between geographically isolated populations versus infrequent mixis of genotypes within populations is more difficult to determine. We examined the g...

P121R25 is a Tn5-induced mutant of the effective Rhizobium leguminosarum bv. phaseoli strain P121R that is unable to use glutamate as the sole carbon and nitrogen source and is defective in symbiotic nitrogen fixation. Enzymatic analysis showed that three enzymes implicated in glutamate metabolism (glutamate dehydrogenase, 2-oxoglutarate dehydrogenase, and glutamate synthase) were affected by t...

abstractin order to investigate the effects of the inoculation with p. putida, r. leguminosarum bv. phaseoli and f. mosseae on yield and nutrient uptake of common bean (phaseolus vulgaris l.), a greenhouse experiment in a completely randomized factorial design with 3 treatment including a: microorganism in four level (no inoculation, p. putida, f. mosseae and mix inoculation of fungi and bacter...

B ernhard Epping, A lexander P. H ansen, Bahm an Djalali and Peter M artin Institut für Pflanzenernährung (330), Universität Hohenheim, Fruwirthstraße 20, D-70593 Stuttgart, Bundesrepublik Deutschland Z. Naturforsch. 49c, 343-351 (1994); received February 4, 1994 Phaseolus vulgaris L., Plant x Bacterium Interaction, Rhizobium leguminosarum bv. phaseoli, Rhizobium tropici, Symbiotic Effectivity ...

Dot immunablot assay was used for determination of nodules produced on soybean. french bean. pigeon pea and urdbean by Bradyrhizobiumjaponicum USDA-I 10; R. leguminosarum bv. phaseoli FB-77 and N-3; Rhizobium sp. A-3 and U-I. respectively. Nodule occupancy by inoculated strains as determined by the test ranged between 73 to 93%. Replica inmrunoblot assay reduced the time required for enumeratio...