نتایج جستجو برای: respectively. seven cell cultures were tested for bvdv contamination using elisa

تعداد نتایج: 12497734  

Journal: :تحقیقات دامپزشکی 0
سید علی قرشی گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری مرتضی دلیری جوپاری گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری دینا مرشدی گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری ترانه حاجیان گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری محسن لطفی موسسه تحقیقات واکسن و سرم سازی رازی کرج

in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - پژوهشکده علوم انسانی و اجتماعی 1391

abstract the present study was conducted to investigate the effect of using model essays on the development of writing proficiency of iranian pre-intermediate efl learners. to fulfill the purpose of the study, 55 pre- intermediate learners of parsa language institute were chosen by means of administering proficiency test. based on the results of the pretest, two matched groups, one as the expe...

2004
H. DVORAKOVA L. VALICEK M. REICHELOVA

Contamination of cell cultures and sera used for animal virus propagation with mycoplasmas represents a serious problem, especially in virology. Therefore specific control measures must be used. To achieve this we introduced PCR for the detection of mycoplasma species in cell cultures and compared its results with ELISA and microbiological culture. Seven mycoplasma species which are the most co...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه رازی - دانشکده علوم 1389

fluorescence chemical sensors for the highly sensitive and selective determination of pb2+ , hg2+, co2+ and fe3+ ions in aqueous solutions are described. the ion sensing system was prepared by incorporating lipophilic ligand (l) as a neutral ion-selective fluoroionophore in the plasticized pvc membrane containing sodium tetraphenylborate or potasium tetrakis (p-chlorophenyl) borate as a liphoph...

پایان نامه :دانشگاه تربیت معلم - سبزوار - دانشکده علوم انسانی و پایه 1393

burnout is a response to the chronic work stress which is prevalent mostly among the people who do people job, like teaching. the purpose of this study was to develop a valid and reliable instrument that can measure burnout in foreign language teachers. although some widely used instruments were developed before which measured burnout in teachers, a specific instrument which include specific sy...

Journal: :New Zealand veterinary journal 2007
F I Hill M P Reichel R J McCoy D J Tisdall

AIM To assess the ability of two commercial bovine viral diarrhoea (BVD) virus (BVDV) antigen-capture enzyme-linked immunosorbent assays (ELISAs) to detect virus in serum and skin biopsies. METHODS Thirty cattle persistently infected (PI) with BVDV were identified using routine diagnostic laboratory testing. Additional ear-notch skin biopsies and blood samples were collected from these animal...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیخ بهایی - دانشکده زبانهای خارجی 1391

abstract pragmatics is the study of communicative action in its sociocultural context. communicative action includes not only using speech acts (such as apologizing, complaining, complimenting, and requesting), but also engaging in different types of discourse and participating in speech events of varying length and complexity. the present study aimed to investigate the assessment of pragm...

ژورنال: تحقیقات دامپزشکی 2007

In order to investigate the prevalence of infection with bovine viral diarrhoea virus(BVDV) of cattle in Ahvaz, blood samples were collected from jugular vein of 572 cattle(521 female, 51 male) from industrial and nonindustrial farms. Sera were stored at -20?C while waiting for analysis. The sera were tested by ELISA, using a commercial ELISA kit for detection of specific antibody to bov...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه رازی - دانشکده علوم 1388

abstract sensitive and precise voltammetric methods for the determination of trace amounts of furaldehydes, mainly as furfural (f) and 5-hydroxymethyl-2-furaldehyde (hmf), in waste waters and other matrices is described. determination of total furaldehyde at < ?g g-1 levels in alkaline buffered aqueous media was individually investigated. by the use of ordinary swv and adsorptive square wave ...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2005
Todd E Cornish Alberto L van Olphen Jacqueline L Cavender Joan M Edwards Paula T Jaeger Leslie L Vieyra Lynn F Woodard Dan R Miller Donal O'Toole

Two techniques performed on skin biopsy samples (ear notches), immunohistochemistry (IHC) and antigen-capture ELISA (AgELISA), were compared for detection of bovine viral diarrhea virus (BVDV) persistent infection (PI) in 559 Angus calves between the ages of 1 and 5 months. The calves also were tested for BVDV infection using virus isolation (VI) and reverse transcription (RT)-PCR on buffy coat...

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