نتایج جستجو برای: ptgs؛ gene
تعداد نتایج: 1141428 فیلتر نتایج به سال:
Post-transcriptional gene silencing (PTGS) is an important mechanism for regulation of plant gene expression and virus-plant interactions. To better understand this process, the heavy metal cadmium was identified as a specific inhibitor in two different PTGS systems, constitutive and inducible. The pattern of cadmium-induced inhibition of PTGS allowed several insights into PTGS development. Fir...
Amplicon transgenes from potato virus X (PVX) are based on a modified version of the viral genome and are efficient activators of post-transcriptional gene silencing (PTGS). To determine whether PVX amplicons activate PTGS in Arabidopsis, we used constructs based on the genome of PVX carrying a green fluorescent protein (GFP) reporter gene. Our analysis of the transgene phenotype exploited prev...
ژن خاموشی پس از ترا نویسی (post-transcriptional gene silencing =ptgs) به عنوان یک مکانیسم طبیعی که به وسیله آن گیاهان اسیدهای نوکلئیک بیگانه مانند ژنوم ویروس ها را شناسایی و تجزیه می کنند مطرح است. در این مطالعه فرض شد که در مقاومت سیب زمینی همسانه g8107(1) در برابر تجمع plrv نیز چنین مکانیسمی دخالت دارد. این فرضیه با آزمایش انتقال ویروس از طریق پیوند آزمون شد. در این آزمایش قطعاتی از ساقه ها...
ژن خاموشی پس از ترا نویسی (post-transcriptional gene silencing =ptgs) به عنوان یک مکانیسم طبیعی که به وسیله آن گیاهان اسیدهای نوکلئیک بیگانه مانند ژنوم ویروس ها را شناسایی و تجزیه میکنند مطرح است. در این مطالعه فرض شد که در مقاومت سیب زمینی همسانه g8107(1) در برابر تجمع plrv نیز چنین مکانیسمی دخالت دارد. این فرضیه با آزمایش انتقال ویروس از طریق پیوند آزمون شد. در این آزمایش قطعاتی از ساقهها...
BACKGROUND RNA-DEPENDENT RNA POLYMERASE6 (RDR6) and SUPPRESSOR of GENE SILENCING 3 (SGS3) are required for DNA methylation and post-transcriptional gene silencing (PTGS) mediated by 21-nt siRNAs produced by sense transgenes (S-PTGS). In contrast, RDR2, but not RDR6, is required for DNA methylation and TGS mediated by 24-nt siRNAs, and for cell-to-cell spreading of IR-PTGS mediated by 21-nt siRN...
Two distinct gene-silencing phenomena are observed in plants: transcriptional gene silencing (TGS), which involves decreased RNA synthesis because of promoter methylation, and posttranscriptional gene silencing (PTGS), which involves sequence-specific RNA degradation. PTGS is induced by deliberate [1-4] or fortuitous production (R.v.B., unpublished data) of double-stranded RNA (dsRNA). TGS coul...
Gene silencing can occur either through repression of transcription, termed transcriptional gene silencing (TGS), or through mRNA degradation, termed post-transcriptional gene silencing (PTGS). Initially, TGS was associated with the regulation of transposons through DNA methylation in the nucleus, whereas PTGS was shown to regulate virus infection through double-stranded RNA in the cytoplasm. H...
Development of post-transcriptional gene silencing (PTGS) agents for therapeutic purposes is an immense challenge in modern biology. Established technologies used to knockdown a specific target RNA and its cognate protein: antisense, ribozyme, RNAi, all conditionally depend upon an initial, critical annealing event of the PTGS ligand to a target RNA. In this review we address the nature of the ...
Although post-transcriptional gene silencing (PTGS) has been studied for more than a decade, there is still a gap in our understanding of how de novo silencing is initiated against genetic elements that are not supposed to produce double-stranded (ds)RNA. Given the pervasive transcription occurring throughout eukaryote genomes, we tested the hypothesis that unintended transcription could produc...
In transgenic and nontransgenic plants, viruses are both initiators and targets of a defense mechanism that is similar to posttranscriptional gene silencing (PTGS). Recently, it was found that potyviruses and cucumoviruses encode pathogenicity determinants that suppress this defense mechanism. Here, we test diverse virus types for the ability to suppress PTGS. Nicotiana benthamiana exhibiting P...
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