We have identified a gene (PslA) that is expressed throughout Dictyostelium development and encodes a novel protein that is required for proper aggregation and subsequent cell-type differentiation and morphogenesis. pslA null (pslA-) cells produce large aggregation streams under conditions in which wild-type cells form discrete aggregates. Tips form along the stream, elongate to produce a finge...

The psl gene cluster, comprising 15 cotranscribed genes from Pseudomonas aeruginosa, was recently identified as being involved in exopolysaccharide biosynthesis and biofilm formation. In this study, we investigated the regulation of the psl gene cluster and the function of the first gene in this cluster, the pslA gene. PslA shows strong similarities to UDP-glucose lipid carriers. An isogenic ma...

BACKGROUND Pseudomonas aeruginosa is considered as a major cause of hospital-acquired infections due to its high antibacterial resistance. Biofilm formation is a well-known pathogenic mechanism in P. aeruginosa infections, since sessile bacteria are protected in an extracellular matrix of exopolysaccharide. The expression of polysaccharide synthesis locus (pslA gene) can be important for biofil...

conclusions the findings of the present study well demonstrated that the p. aeruginosa biofilm-producing isolates were more resistant to the tested antibiotics. furthermore, because of wide distribution, it seems that the psla gene is associated with biofilm formation. results biofilm formation was observed in 70% of the p. aeruginosa isolates. the potential formation of biofilm was significant...

BACKGROUND Pseudomonas aeruginosa is an important nosocomial pathogen characterized by its innate resistance to multiple antimicrobial agents. Plasmid-mediated drug resistance also occurs by the production of extended-spectrum β-lactamases (ESBL), metallo β-lactamases (MBL), and AmpC β-lactamases. Another important factor for establishment of chronic infections by P. aeruginosa is biofilm forma...

objective(s): bacterial biofilm formation causes many persistent and chronic infections. the matrix protects biofilm bacteria from exposure to innate immune defenses and antibiotic treatments. the purpose of this study was to evaluate the biofilm formation of clinical isolates of pseudomonas aeruginosa and the activity of zinc oxide nanoparticles (zno nps) on biofilm. materials and methods: aft...

conclusions biofilm formation (both strong and weak) strongly correlated with psla gene carriage. biofilm formation also correlated with mbl and ampc β-lactamase production. more importantly, multiple-β-lactamase phenotype was associated with formation of strong biofilms. results biofilm formation was observed in 43.5% of the isolates, of which 66.7% produced strong and 33.3% formed weak biofil...

Objective(s): Bacterial biofilm formation causes many persistent and chronic infections. The matrix protects biofilm bacteria from exposure to innate immune defenses and antibiotic treatments. The purpose of this study was to evaluate the biofilm formation of clinical isolates of Pseudomonas aeruginosa and the activity of zinc oxide nanoparticles (ZnO NPs) on biofilm. Materials and Methods: Aft...

BACKGROUND Aortic stenosis (AS) is a common valve problem that causes significant morbidity and mortality. The goal of this study was to determine whether an emergency physician (EP) could determine severe AS by reviewing only two B-mode echocardiographic views (parasternal long axis (PSLA) and parasternal short axis (PSSA)) obtained by trained echocardiographers. METHODS A convenience sample...