An improved polymerase chain reaction (PCR)-based method for determining the species composition of Eimeria in poultry litter was developed by incorporating species-specific internal standards in the assay. Internal standard molecules were prepared by fusing seven different Eimeria species-specific intervening transcribed sequence 1 (ITS1) rDNA primer pairs to a non-Eimeria DNA molecule and by ...

A method was developed to recover Eimeria spp. oocysts directly from poultry litter and determine which species of Eimeria were present using polymerase chain reaction (PCR) based on the ITS1 rDNA sequence. The species composition of Eimeria oocysts was also compared before and after propagation in susceptible chickens to determine if the relative proportion of each species changed after expans...

BACKGROUND Chicken is fast becoming the world's most consumed meat. As a consequence poultry health is more important now than ever before, with pathogens of chickens recognised as serious threats to food security. One such threat are Eimeria species parasites, protozoa which can cause the disease coccidiosis. Eimeria can compromise economic poultry production and chicken welfare, and have seri...

background: coccidiosis of domestic fowl, caused by species of the genus eimeria , is responsi­ble for important economic losses in poultry production. because different species and/or strains can vary in pathogenicity and other biological parameters, their precise characteriza­tion is important for epizootiological studies. methods: fifty samples from litter, whole intestinal tract and feces w...

background: coccidian infection in poultry is the most common infection in the world and is the cause of mortality and great economic losses in poultry production. objectives: this study was aimed to determine the frequency and eimeria species diversity in broiler farms of hamadan suburb, iran. methods: a total of 16 broiler farms were randomly selected with different production age in 2012. th...

Roles of probiotic microorganisms in digestive physiology are contributed to enhancing animals health and reducing the incidence of gastrointestinal diseases. Banning the use of antibiotics as growth promoters in poultry in many countries has been contributed to the increasing use of probiotics because long-term use of antibiotics causes antibiotic resistance of pathogenic bacteria in poultry a...

Chicken coccidiosis, caused by Eimeria sp., occurs in almost all poultry farms and causes huge economic losses in the poultry industry. Although this disease could be controlled by vaccination, the reduced feed conservation ratio limits the widespread application of anticoccidial vaccines in broilers because some intermediate and/or low immunogenic Eimeria sp. only elicit partial protection. It...

background: the control of coccidiosis in poultry industryis dominated by prophylactic chemotherapy; however,drug resistance is a serious problem. alternative control methodssuch as vaccination have been accepted as a practical method forcontrolling coccidiosis in chickens. considering the immunologicalvariation of eimeria strains, the efficacy of live coccidiosisvaccines may be compromised. ob...

Drug resistance is a problem wherever livestock are raised under intensive conditions and drugs are used to combat parasitic infections. This is particularly true for the anticoccidial agents used for the prevention of coccidiosis caused by protozoa of the apicomplexan genus Eimeria in poultry. Resistance has been documented for all the dozen or so drugs approved for use in chickens and varying...

Small-scale commercial poultry production is emerging as an important form of livestock production in Africa, providing sources of income and animal protein to many poor households, yet the occurrence and impact of coccidiosis on this relatively new production system remains unknown. The primary objective of this study was to examine Eimeria parasite occurrence on small-scale commercial poultry...