The potential of two small polyL -lysines (sPLLs), low molecular weight sPLL (LMW-L) containing 7–30 lysine residues and L18 with 18 lysine repeats, to enhance the efficiency of liposome-mediated gene transfer (GT) with cationic lipid DOCSPER [1,3-dioleoyloxy-2-(N 5 -carbamoyl-spermine)-propane] in vascular smooth muscle cells (SMCs) was investigated. Dynamic light scattering was used for deter...

In a very recent paper published in November 2017 in the peer-reviewed scientific journal Bio Accent Open Access HIV, I describe a novel procedure designed to improve efficacy of DNA vaccines in areas as diverse as Alzheimer’s disease, HIV infection and cancer [1]. This procedure is based on non-covalent binding of highly charged molecules to DNA; positively charged polyL-lysine is first bound ...

In recent years, many applications have been developed for the detection of different toxic metals (As, Cd, Cu, Hg, Ni, Pb) in water samples. The classic analytical methods (ICP-MS, AAS with graphite furnace, ICP-EOS ultrasonic nebulizer) not only require a longer analysis time (pretreatment sample and analysis), but also costs involved are higher as result expensive equipment, associated metho...

The effects of deoxyribonucleoprotein, histories, and polyL-lysine on amino acid uptake and incorporation into protein in Walker 256 tumor cells have been investigated. When Walker 256 tumor cells were incubated with deoxyribonucleoprotein, histories, or poly-L-lysine there was a reduction in the amount of 14C-labeled amino acids found in the hot 5% trichloroacetic acid-insoluble fraction. The ...

luid shear stress (FSS) induces many forms of responses, including phosphorylation of extracellular signal– regulated kinase (ERK) in endothelial cells (ECs). We have earlier reported rapid tyrosine phosphorylation of platelet endothelial cell adhesion molecule-1 (PECAM-1) in ECs exposed to FSS. Osmotic changes also induced similar PECAM-1 and ERK phosphorylation with nearly identical kinetics....

Type Ib diamonds emit bright fluorescence at 550-800 nm from nitrogen-vacancy point defects, (N-V)(0) and (N-V)(-), produced by high-energy ion beam irradiation and subsequent thermal annealing. The emission, together with noncytotoxicity and easiness of surface functionalization, makes nano-sized diamonds a promising fluorescent probe for single-particle tracking in heterogeneous environments....

Primary bovine osteoblast cell cultures were used to study the response of osteoblasts ton three different biomaterials, ionomeric cement (IC), tri-calcium phosphate poly-L-lactic acid composite foil (TCP) and polyL-lactate-polycitric acid composite foil (PLA). The cells which grew on the materials produced all typical bone matrix proteins and were osteoblast-Iike as shown by immuno-staining. A...

Runtime verification techniques are increasingly being applied in industry as a lightweight formal approach to achieve added assurance of correctness at runtime. A key issue determining the adoption of these techniques is the overheads introduced by the runtime checks, affecting the performances of the monitored systems. Despite advancements in the development of optimisation techniques lowerin...