Time-resolved fluorescence of 4',6-diamidino-2-phenylindole (DAPI) complexes show that for a homogeneous polymer (polyd(AT) or polyd(A).polyd(T)) at high P/D (phosphate/dye) ratio, a single exponential component adequately describes the fluorescence decay. For the AT polymers at low P/D ratio or for native DNA, the decay cannot be described by a single-exponential term. A continuous distributio...

The standard dispatching mechanisms built into programming languages sometimes fail to satisfy the needs of the programmer. In the case of Java, the need for more flexibility has led to the development of a number of tools, including visitors and multi-method extensions, that each add some particular functionality, but lack the generality necessary to support user-defined dispatching mechanisms...

The conformation of DNA's with adenine-thymine tracts exhibiting retardation in electrophoretic migration and considered as curved were investigated in solution by CD and RAMAN spectroscopy. The following curved multimers with adenine tracts but of different flanking sequences d(CA5TGCC)n, d(TCTCTA6TATATA5)n, d(GA4T4C)n yield CD spectroscopic features indicating a non-B structure of the dA.dT t...

Further mapping of the mouse T-cell factor specific for poly(Try,Glu)-polyD-LAla--polyLys is reported. It is shown to be a product of the I-A subregion of the H-2 complex by the use of antisera either raised specifically against or made specific, by absorption, for different regions of the H-2 complex. The factor cooperates across allogeneic barriers, e.g., when factor produced by one strian is...

We have measured the thickness profiles of ultrasmall (picoliter volume) droplets of polyd~mcthvls~loxane during thelr spreading on a silver surface at centra! thicknesses below one monolayer. The resulting prufile shapes can he approximated well by shperical caps, and the diffusion dynamics are q u ~ t e difterent from those expected for mintlike particles. Thc cxpcrimental data are in remarka...

The c o n d e n s a t io n o f racemic ly s in e to p o ly -D L ly s in e produces a great number o f d is t in ­ guishable molecules. Following merely statistical reasoning the number o f “pure peptides“ — consisting of only one sort of enantiomers — should be extremely low. The hydrolysis of polyD L -ly s in e by pronase a n d t a k a -d ia s ta s e shows that a content of about 3% “pure“ pol...

Detailed models are presented for the triple-stranded polynucleotide helices of poly (U)-poly (A)-poly (U) (two forms), poly (U)-poly d (A) -poly (U), poly d(C)-poly d(I)-poly d(C), poly d(T)-polyd(A)-poly d(T) and poly (I)-poly (A)-poly (I). The models were genrated using a computerized, linked-atom procedure which preserves standard bond lengths, bond anglesand sugar ring conformations, const...

Let fλ : P 1 → P be a family of rational maps of degree d > 1, parametrized holomorphically by λ in a complex manifold X. We show that there exists a canonical closed, positive (1,1)-current T on X supported exactly on the bifurcation locus B(f) ⊂ X. If X is a Stein manifold, then the stable regime X − B(f) is also Stein. In particular, each stable component in the space Polyd (or Ratd) of all ...

The MAPK signal transduction cascade is dysregulated in a majority of human tumors. Here we report that a nanoparticle-mediated targeting of this pathway can optimize cancer chemotherapy. We engineered nanoparticles from a unique hexadentate-polyD,L-lactic acid-co-glycolic acid polymer chemically conjugated to PD98059, a selective MAPK inhibitor. The nanoparticles are taken up by cancer cells t...

In the routine treatment of coronary artery disease, balloon angioplasty has been replaced by stents. The use of bare-metal stents resulted in lower rates of restenosis and repeat revascularisations. Drug-eluting stents (DES), designed with thinner struts and anti-proliferative drugs, have further enhanced the efficacy and safety of percutaneous coronary intervention. Nevertheless, the new gene...