3-Phenyllactic acid (PhLA) is a broad spectrum antimicrobial compound active against bacteria and fungi. PhLA exists in two chiral isomers, L-PhLA and D-PhLA which show antimicrobial activity. D-PhLA shows more antimicrobial activity than L-PhLA and hence it is receiving great attention as food and feed additive in place antibiotics which control the microbial contamination and thereby increase...

Plasma postheparin lipolytic activity (PHLA) was measured on 50 patients with systemic lupus erythematosus (SLE). Plasma PHLA was significantly decreased in SLE patients. This decrease was most striking in the acute phase of the disease. There was a close relationship between decreased PHLA and immunologic factors indicative of the acute phase of SLE. These immunologic factors included shaggy a...

It is widely assumed that measurement of lipolytic activity in post-heparin plasma (PHLA) reflects the activity of the enzyme lipoprotein lipase in extrahepatic tissues, i.e. adipose tissue, muscle and the heart. Apart from the findings of low PHLA in patients with Fredrickson’s type I (fat-induced) hyperlipoproteinaemia (Fredrickson, Ono & Davis, 1963), in which a defect in tissue lipoprotein ...

Human CD8+ cytotoxic T lymphocytes (CTLs) are known to play an important role in tumor control. In order to carry out this function, the cell surface-expressed T-cell receptor (TCR) must functionally recognize human leukocyte antigen (HLA)-restricted tumor-derived peptides (pHLA). However, we and others have shown that most TCRs bind sub-optimally to tumor antigens. Uncovering the molecular mec...

The antimicrobial metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) contributes to the capacity of Pseudomonas fluorescens strain CHA0 to control plant diseases caused by soilborne pathogens. A 2, 4-DAPG-negative Tn5 insertion mutant of strain CHA0 was isolated, and the nucleotide sequence of the 4-kb genomic DNA region adjacent to the Tn5 insertion site was determined. Four open reading frames ...

The concept of algae biorefinery is attracting attention because the abundant valuable compounds within algal biomass. Phenyllactic acid (PhLA), a broad-spectrum antimicrobial organic that can be produced by lactic bacteria (LAB), considered potential biopreservative. In this study, cascading approach was developed to harvest PhLA from Porphyra residues LAB fermentation. strains were cultivated...

A 5-yr old male proband and his sister have had hypertriglyceridemia and hepatosplenomegaly since birth. When studied on a metabolic ward, they demonstrated rapid decreases in serum triglycerides on 3 g fat/day diets. Oral glucose tolerance tests were normal. Postheparin lipolytic activity (PHLA) against chylomicrons was virtually absent in both children whereas the mother and a normolipemic si...

BACKGROUND Pseudomonas fluorescens 2P24 is a rhizospheric bacterium that aggressively colonizes the plant roots. It produces the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), which contributes to the protection of various crop plants against soil borne diseases caused by bacterial and fungal pathogens. The biosynthesis of 2,4-DAPG is regulated at the transcriptional level in the expression o...

The rise in plasma triglyceride (TG) levels associated with estrogen administration has been thought to arise from impaired clearance because of the uniform suppression of post-heparin lipolytic activity (PHLA). Recently PHLA has been shown to consist of two activities: hepatic TG lipase and extrahepatic lipoprotein lipase (LPL). To determine whether estrogen might induce a selective decline in...

The intravascular injection of heparin results in the rapid appearance in the blood of a lipolytic enzyme capable of clearing lipemic sera and hydrolyzing artificial triglyceride emulsions (1, 2). This enzyme has been termed clearing factor (3), lipoprotein lipase (4), clearing factor lipase (2), or postheparin plasma lipolytic activity (PHLA) (5). A lipolytic enzyme with characteristics nearly...