OBJECTIVE To construct and identify multi-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2. METHOD Primers were designed according to the gene sequences of restriction enzyme cutting site of recombinant pcDNA3-p30-ROP2 and hepatitis B surface antigen (HBsAg). The target fragment of HBsAg was amplified and cloned to expression vector pcDNA3-p30-ROP2 by restriction enzyme digestion and ...

background: cervical cancer has been shown to be highly associated with human papillomavirus (hpv) infection. the viral oncogenes e6 and e7 are constantly expressed by the tumor cells and are therefore potent targets for therapeutic genetic vaccination. in the present study, it was investigated the potential effect of hpv-16 e6, e7 and l1 co-administration to activate specific cytotoxic t lymph...

To observe the in vitro expression of DNA vaccine pcDNA3-Sj22.7 and its immunological effect in mice, the recombinant plasmid pcDNA3-Sj22.7 was used to transfect HeLa cells with liposome-mediated method and the expression of Sj22.7 mRNA and protein was examined using reverse transcription-polymerase chain reaction, sodium dodecylsulfate-polyacrylamide gel electrophoresis and Western blot. Then,...

Fig. I. Survivin inhibits Baxand Faa-inducedapoptosis in 293 cells. A, 293 cells were transfectedwith pcDNA3myc (control), pcDNA3myc-Survivin,pcDNA3myc-XIAP. pcDNA3-Bax, pcDNA3myc-Survivin+ pcDNA3-Bax, or pcDNA3myc-XIAP+ pcDNA3-Bax plasmidDNAs. One day later,the percentageof apoptoticcells (DAPI-staining)was determined (mean ±SE for three determinations). B, the expression of the transfected p...

We explored whether p53 upregulated modulator of apoptosis (PUMA) gene transfection could enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells. The liposome-mediated recombinant eukaryotic expression vector PU-MA-pCDNA3 and empty vector plasmid were stably transfected into MCF-7 cells. Epirubicin (0.01-100 μM) was applied to MCF-7, MCF-7/PUMA, and MCF-7/pCDNA3 ce...

Fig. I. Survivin inhibits Baxand Faa-inducedapoptosis in 293 cells. A, 293 cells were transfectedwith pcDNA3myc (control), pcDNA3myc-Survivin,pcDNA3myc-XIAP. pcDNA3-Bax, pcDNA3myc-Survivin+ pcDNA3-Bax, or pcDNA3myc-XIAP+ pcDNA3-Bax plasmidDNAs. One day later,the percentageof apoptoticcells (DAPI-staining)was determined (mean ±SE for three determinations). B, the expression of the transfected p...

We demonstrate the use of combination therapy to overcome the limitations of cancer DNA vaccines by adding radioiodine gene therapy in an animal cancer model. We established a stable cell line (CT26/hMUC1-hNIS-Fluc: CMNF) expressing the hMUC1, hNIS and Fluc genes using a retro- and lentivirus system. The survival rates (%) of CMNF cells were determined using clonogenic assays after (131)I treat...

BACKGROUND Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. METHODS In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. RESULTS In vitro and in vivo studies showed that B cells efficiently pres...

The zinc-fingers and homeoboxes protein 1 (ZHX1) consists of 873 amino acid residues, is localized in the cell nucleus and appears to act as a transcriptional repressor. Previous studies have shown that ZHX1 interacts with nuclear factor Y subunit α (NF-YA), DNA methyltransferases (DNMT) 3B and ZHX2, all of which are involved in tumorigenesis. However, the exact role of ZHX1 in tumorigenesis re...

In this study, we examined the immunogenic properties of the Leishmania infantum acidic ribosomal protein P0 (LiP0) in the BALB/c mouse model. The humoral and cellular responses induced by the administration of the LiP0 antigen, either as soluble recombinant LiP0 (rLiP0) or as a plasmid DNA formulation (pcDNA3-LiP0), were determined. Also, the immunological response associated with a prime-boos...