Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...

background: the purpose of this study was to clone, express, and purify a novel multidomain fusion protein of micobacterium tuberculosis (mtb) in a prokaryotic system. methods: an hspx/esxs gene construct was synthesized and ligated into a pgh plasmid, e. coli top10 cells were transformed, and the vector was purified. the vector containing the construct and pet-21b (+) plasmid were digested wit...

Background Environmental and cellular inappropriate conditions can cause damages to cells nucleotide poll. Deamination and oxidation damages interfere with cell�s vital reactions. Inosine triphosphate pyrophosphatase (ITPA), an evolutionary conserved enzyme, plays a critical role in elimination of non-canonical bases. In human genome, the ITPA gene is located on chromosome 20 short arm and tran...

background: hepatitis c virus (hcv) is a main public health problem causing chronic liver infection and subsequently liver cirrhosis and lethal hepatocellular carcinoma (hcc). vaccination based on hcv capsid proteins has attracted a special interest for prevention of viral infections. the core protein is a basic and evolutionary most conserved protein, which regulates the cellular processes rel...

the gene encoding the human basic fibroblast growth factor (hbfgf) has been already chemically-synthesized and cloned in pet-3a expression vector (pasteur institute of iran). in the present study, we compared the level of expression of this synthetic hbfgf and its related cdna in escherichia coli. the pbr322-cdna of hbfgf supplied by dr. seno (from molecular biology dept, okaido prefectural uni...

Background: Hepatitis C virus (HCV) is a main public health problem causing chronic liver infection and subsequently liver cirrhosis and lethal hepatocellular carcinoma (HCC). Vaccination based on HCV capsid proteins has attracted a special interest for prevention of viral infections. The core protein is a basic and evolutionary most conserved protein, which regulates the cellular processes rel...

Background: Rotavirus group A (RVA) is recognized as a major cause of severe gastroenteritis in children and new-born animals. Nonstructural protein 4 (NSP4) is responsible for the enterotoxic activity of these viruses in the villus epithelial cells. Amino acids 114-135 of NSP4 are known to form the diarrhea-inducing region of this viral enterotoxin. Therefore, developing an NSP4 lacking the en...

Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells  has been known as a major mediator in both  adaptive and native immune system  due to a board range of effects on  different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...

The gene encoding the human basic fibroblast growth factor (hbFGF) has been already chemically-synthesized and cloned in pET-3a expression vector (Pasteur Institute of Iran). In the present study, we compared the level of expression of this synthetic hbFGF and its related cDNA in Escherichia coli. The pBR322-cDNA of hbFGF supplied by Dr. Seno (from Molecular Biology Dept, Okaido prefectural uni...