A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...

Restriction mapping is one of the essential steps in gene analysis and molecular biology studies. Slab gel electrophoresis is the traditional way to separate DNA fragments for restriction mapping. However, slab gel electrophoresis does not provide sufficient resolution as required in many mapping applications, and the use of radioisotopes in traditional mapping methods creates health hazards. I...

New complexes and adducts of xanthate the general formula [M(cyclopentyl xant.)2] xant.)2.nL] Where M= Mn(ΙΙ) Fe(ΙΙ), Co(ΙΙ), Ni(ΙΙ), Cu(ΙΙ) Zn(ΙΙ), (cyclopentyl xant.)2=[Cyclopentyl ligand], n=2 L= Pyridine, 3-acetyl pyridine & Quinoline n=1, ethylenediamine, (1,10)-phenanthroline, prepared has been evaluated based on their magnetic, electrical, physical characteristics. And spectral metho...

Source and Description: Cos39 cosmid was obtained from a chromosome 22-enriched library and its partial sequence revealed a zinc finger motif of the Kruppel type (1). A 1 kb Sau3A I fragment derived from Cos39 and giving a positive signal when hybridized with a poly(dC-dA)-poly(dG-dT) probe was subcloned into the BamHI site of pBluescript SK+ (Stratagene). Partial sequence of this subclone reve...

Cloning techniques are fundamental to molecular biology. Classically, recombinant plasmid and/or phage vectors are prepared in vitro, the transformation step only serving for amplification purposes. A different approach would exploit the natural intracellular enzymatic machinery to produce recombinant DNA molecules. Such techniques are well-known to mutagenize chromosomal DNA by e.g. transposon...

An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR clon...

Background: The quantitative assays are important tests in the management of patients with HIV-1/AIDS. The important step in developing the assay is the availability of the standard HIV-1 RNA. For this purpose, we optimized in vitro HIV-1 RNA transcription to produce the standard HIV-1 RNA. Methods: The HIV-1 DNA was amplifi ed from pNL43 by PCR using a primer pair that was specifi c for conser...

To study possible effects of radiation and chemical pollutants on native rodents in highly polluted sites of the former Soviet Union we developed a system of microsatellite markers in mice (Apodemus). Microsatellites can provide information on mutation rate and population structure of the affected animals. Dubrova et al. (1996) reported that mutation rates at minisatel-lite loci in humans who l...

Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His− TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2...