materials and methods the subjects included 27 male and 3 female patients with advanced chronic periodontitis. the mean age of the patients was 47.6 ± 2.0 years old. p. gingivalis was isolated from the samples and identified by various diagnostic tests, including gram staining, indol test, and fluorescent test. minimum inhibitory concentration (mic) of the essential oil against isolated p. ging...

It is well known that strain and virulence diversity exist within the population structure of Porphyromonas gingivalis. In the present study we investigate intra- and inter-species variability in biofilm formation of Porphyromonas gingivalis and partners Prevotella intermedia and Prevotella nigrescens. All strains tested showed similar hydrophobicity, except for P. gingivalis W83 which has roug...

The capsular polysaccharide (CPS) of the periodontal pathogen Porphyromonas gingivalis is an important virulence factor for this organism. We purified P. gingivalis CPS, immunized mice with this antigen, and assessed the vaccine potential of P. gingivalis CPS by using the murine oral challenge model. Animals immunized with P. gingivalis CPS developed elevated levels of immunoglobulin M (IgM) an...

Background and Aim: This study aimed to assess the antibody titer against Porphyromonas gingivalis (P. gingivalis) in rats with experimentally induced periodontitis. Materials and Methods: Thirty-three Surrey rats were randomly divided into three groups (n=11). Group 1 was vaccinated with formalin-killed whole cell (FKWC) P. gingivalis with incomplete Freund's adjuvant as vaccine carrier and o...

conclusions p. gingivalis and p. intermedia could play a role in implant failure by changing the expression profiles of genes related to bone formation and resorption. results viability was reduced to 56.8% (p. gingivalis) and 52.75% (p. intermedia) at 1000 multiplicity. amongst 48 genes examined, expressions of bmper, smad1, il8 and nfrkb were found to be highly upregulated by both bacterial c...

OBJECTIVES The aim of this study was to determine whether recurrent intravenous injections with Porphyromonas gingivalis (P gingivalis), mimicking periodontitis-associated bacteremia, promotes coronary artery and aortic atherosclerosis in pigs. METHODS AND RESULTS Pigs (n=36) fed low- or high-fat chow were divided into P gingivalis-sensitized and P gingivalis-challenged groups or P gingivalis...

Induction of mucosal immune responses against Porphyromonas gingivalis within the oral cavity of dogs was studied by immunizing with pH-sensitive fusogenic polymer (MGluPG)-modified liposome-associated cell lysate. Dogs immunized with P. gingivalis cell lysate-containing MGluPG-modified liposomes by intraocular (eye drop) route displayed significant levels of P. gingivalis cell lysate-specific ...

Background: The mouth cavity hosts various types of anaerobic bacteria including Porphyromonas gingivalis, which causes periodontal inflammatory diseases. P. gingivalis is a gram-negative oral anaerobe and is considered as a main etiological factor in periodontal diseases. Several studies have reported a relationship between P. gingivalis in individuals with periodontal diseases and a critical ...

OBJECTIVE Although the exact reason is not known, encapsulated gram-negative Porphyromonas gingivalis strains are more virulent than non-encapsulated strains. Since difference in virulence properties may be due to difference in cytokine production following recognition of the bacteria or their products by the host inflammatory cells, we compared cytokine production following stimulation with ba...

Porphyromonas gingivalis is a key pathogen in the polymicrobial biofilm that is associated with the oral disease chronic periodontitis. A number of studies have shown that in humans the level of P. gingivalis in the polymicrobial biofilm is positively correlated with disease progression. The aim of this study was to develop a P. gingivalis diagnostic that has high specificity and sensitivity fo...