نتایج جستجو برای: outer membrane proteins (omp)

تعداد نتایج: 884310  

Journal: :iranian biomedical journal 0
علی شیخیان ali sheikhian زهیر محمد حسن zuhair m. hassan علی مصطفایی ali mustafaie فاضل شکری fazel shokri رضا ملک زاده reza malekzadeh فریده سیاوشی farideh siavoshi

helicobacter pylori infections are accompanied with the release of some antigenic material into the stool. outer membranes proteins (omp) are among the major antigens of bacteria, which in comparison with other bacterial constituents have less cross-reactivity. in this study, omp of h. pylori were isolated from 11 clinical isolates and rabbit antiserum against them was used to detect possible a...

Background: Outer membrane proteins (OMPs) constitute the main structure and about half of the cell wall of Gram-negative bacteria. The OMPs of Escherichia coli (E. coli) play an important role in its drug resistance. Previous studies have shown that the OMPs of E. coli enhance its pathogenic effects by helping the bacterium to evade the immune defense and promote its adsorption to host cells. ...

Journal: :cell journal 0

introduction: legionnair's disease bacterium is a gram negative, facultative intracellular bacilli which parasitize human alveolar macropages as well as some species of free living amobae. this study was achieved in order to compare the cell wall composition in intracellular bacteria. whole cell and outer membrane proteins and the bacterial lipopolysacharide (lps) were analyzec on sds page syst...

BAHMAN TABARAIE, FERESHTEH SHAHCHERAGHI, GHORBAN BEHZADIAN-NEJAD, MOHAMMAD BAGHER ESLAMI,

Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...

ژورنال: :پژوهش در پزشکی 0
زهره عابدینی فر zohreh abediny far department of microbiology, faculty of medicine, shahid beheshti university of medical sciencesگروه میکرب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران فرحنوش دوستدار farahnoosh doustdar department of microbiology, faculty of medicine, shahid beheshti university of medical sciencesگروه میکرب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران فهیمه اسدی آملی fahimeh asadi amoli faculty of medicine, tehran university of medical sciencesدانشکده پزشکی، دانشگاه علوم پزشکی تهران، تهران، ایران حسین گودرزی hossein goudarzi department of microbiology, faculty of medicine, shahid beheshti university of medical sciencesگروه میکرب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران فاطمه فلاح fatemeh fallah department of microbiology, faculty of medicine, shahid beheshti university of medical sciencesگروه میکرب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

سابقه و هدف: کلامیدیا تراکوماتیس یک باکتری داخل سلولی اجباری با اهمیت کلینیکی بالا میباشد که عفونتهای انسانی ناشی از این باکتری ترجیحاً چشمها و دستگاه تناسلی را گرفتار میکنند. کلامیدیا تراکوماتیس، شایعترین عامل کونژنکتیویت فولیکولار مزمن  و عامل 20 درصد موارد کونژنکتیویت حاد می باشد. از آنجاییکه تشخیص سریع می تواند در کاهش عوارض طولانی مدت بیماری موثر باشد در این تحقیق ما بر آن شدیم که روشهای مخ...

Journal: :medical journal of islamic republic of iran 0
fereshteh shahcheraghi from the department of microbiology, medical school, tarbiat modaress university, tehran, mohammad bagher eslami from the department of microbiology, medical school, tarbiat modaress university, tehran, bahman tabaraie the department of bacterial vaccine and antigen production, pasteur institute of iran, tehran, islamic republic of iran. ghorban behzadian-nejad from the department of microbiology, medical school, tarbiat modaress university, tehran,

isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...

Journal: :Infection and immunity 2005
Job E Lopez William F Siems Guy H Palmer Kelly A Brayton Travis C McGuire Junzo Norimine Wendy C Brown

Immunization with purified Anaplasma marginale outer membranes induces complete protection against infection that is associated with CD4+ T-lymphocyte-mediated gamma interferon secretion and immunoglobulin G2 (IgG2) antibody titers. However, knowledge of the composition of the outer membrane immunogen is limited. Recent sequencing and annotation of the A. marginale genome predicts at least 62 o...

Journal: :Journal of bacteriology 2008
Yumi Kumagai Haibin Huang Yasuko Rikihisa

Ehrlichia chaffeensis, an obligatory intracellular gram-negative bacterium, must take up various nutrients and metabolic compounds because it lacks many genes involved in metabolism. Nutrient uptake by a gram-negative bacterium occurs primarily through pores or channels in the bacterial outer membrane. Here we demonstrate that isolated E. chaffeensis outer membranes have porin activities, as de...

Journal: :infection, epidemiology and medicine 0
pejvak khaki national reference laboratory for leptospira, department of microbiology, razi vaccine & serum research institute, karaj, iran soheila moradi bidhendi national reference laboratory for leptospira, department of microbiology, razi vaccine & serum research institute, karaj, iran yung-fu chang department of population, medicine and diagnostic sciences, college of veterinary medicine, cornell university, ithaca, ny, usa maryam sadat soltani national reference laboratory for leptospira, department of microbiology, razi vaccine & serum research institute, karaj, iran kayvan tadaion department of aerobic vaccine production, razi vaccine & serum research institute, karaj, ir iran

background: leptospirosis has been recognized as an important reemerging infectious disease caused by pathogenic leptospira spp. a major challenge of this disease is the application of a basic research to improve diagnostic method. outer membrane proteins of leptospira are potential candidates that could be useful in diagnosis. among them the lipl41 is an immunogenic protein which is present on...

A. Prasad F. Mukherjee G. K. Sharma L. Rajendra S. K. Rana V. A. Srinivasan, V. S. Bahekar

The use of liposome as an adjuvant and a vaccine carrier has been cited previously in the literature. It has also been shown to be effective in enhancing the immunogenicity of vaccine candidates. BALB/c mice immunized subcutaneously with outer membrane protein (OMP) of Brucella abortus S19 vaccine strain entrapped in a commercial cationic liposome (S19-OMP-liposome) for vaccine delivery, showed...

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