Si ngh A, Islam MN (2010) In vitro evaluation of Trichoderma spp against Phytophthora nicotianae. Int. J. Expt. Agric. 1(1), 20-25. The Blank sank disease of tobacco caused by Phytophthora nicotianae results in considerable loss of the crop. This lab study aimed to investigate antagonistic activity of Trichoderma spp isolates against P. nicotianae. Three isolates of Trichoderma harzianum and on...

Phytophthora nicotianae and P. cactorum cause Phytophthora rot of strawberry. A duplex real-time PCR technique for simultaneous detection and quantification of the two pathogens was developed. Species-specific primers for P. nicotianae and P. cactorum were designed based on the internal transcribed spacer regions (ITS) of rDNA and the ras-related protein gene Ypt1, respectively. TaqMan probes w...

Infection of citrus seedlings by Tylenchulus semipenetrans was shown to reduce subsequent infection of roots by Phytophthora nicotianae and to increase plant growth compared to plants infected by only the fungus. Hypothetical mechanisms by which the nematode suppresses fungal development include nutrient competition, direct antibiosis, or alteration of the microbial community in the rhizosphere...

Effect of sewage sludge in vitro to suppress Phytophthora nicotianae Soil organic matter amendments may provide an alternative management practice for soil diseases caused by Phytophthora spp. In this paper we have evaluated the effectiveness of residential sewage sludge to suppress P. nicotianae under laboratory conditions. The effect of sewage sludge on P. nicotianae survival was evaluated by...

Phytophthora nicotianae is a common and destructive pathogen of numerous ornamental, agronomic and horticultural crops such as tomato, tobacco and citrus. Three monobranched C13 alcohol alkoxylate non-ionic surfactants were evaluated for in vitro inhibitory activity against the different asexual structures of P. nicotianae. The same surfactants, labelled MBA1301, MBA1303 and MBA1306, were teste...

The oomycete plant pathogen Phytophthora nicotianae causes diseases on a wide range of plant species. To facilitate isolation and functional characterization of pathogenicity genes, we have constructed a large-insert bacterial artificial chromosome (BAC) library using nuclear DNA from P. nicotianae H1111. The library contains 10,752 clones with an average insert size of 90 kb and is free of mit...

Thailand is one of the largest citrus producers in Southeast Asia. Pathogenic infection by Phytophthora, however, has become one of major impediments to production. This study identified a pathogenic oomycete isolated from rotted roots of pomelo (Citrus maxima) in Thailand as Phytophthora nicotianae by the internal transcribed spacer ribosomal DNA sequence analysis. Then, we examined the in vit...

This study evaluated the types of gene action governing the inheritance of resistance to Phytophthora nicotianae necrosis in populations derived from two crosses involving two susceptible (Beldi and Nabeul II) and one resistant (CM334) cultivars of pepper (Capsicum annuum L.). Populations, composed of Pr, Ps, F(1) , F (2) , BC (1) Pr, and BC (1) Ps generations, were inoculated with six P. nicot...

Phytophthora nicotianae is one of the most destructive plant pathogens affecting a variety of plants, causing black shank of tobacco, among several other devastating diseases. Herein, we assembled the mitochondrial genome of P. nicotianae and analyzed its gene content and genome structure, performed comparative mitochondrial genomics analysis, and assessed phylogenetic relationships among oomyc...

ABSTRACT Phytophthora nicotianae and P. palmivora infect and cause rot of fibrous roots of susceptible and tolerant citrus rootstocks in Florida orchards. The infection and colonization by the two Phytophthora spp. of a susceptible citrus host, sour orange (Citrus aurantium), and a tolerant host, trifoliate orange (Poncirus trifoliata), were compared using light and electron microscopy. Penetra...