Although the mechanisms regulating the formation of embryonic skeletal muscle in vertebrates are well characterized, less is known about postnatal muscle formation even though the largest increases in skeletal muscle mass occur after birth. Adult muscle stem cells (satellite cells) appear to recapitulate the events that occur in embryonic myoblasts. In particular, the myogenic basic helix-loop-...

Although skeletal muscle metabolism is a well-studied physiological process, little is known about how it is regulated at the transcriptional level. The myogenic transcription factor myogenin is required for skeletal muscle development during embryonic and fetal life, but myogenin's role in adult skeletal muscle is unclear. We sought to determine myogenin's function in adult muscle metabolism. ...

Duchenne muscular dystrophy (DMD) is the most prevalent inherited childhood muscle disorder in humans. mdx mice exhibit a similar pathophysiology to the human disorder allowing for an in-depth investigation of DMD. Myogenin, a myogenic regulatory factor, is best known for its role in embryonic myogenesis, but its role in adult muscle maintenance and regeneration is still poorly understood. Here...

Species and Locus. Pig Myogenin (MYOG). Source and Description of Primers. Oligonucleotide primers designed from pig cDNA sequence (GenBank accession no. U14331) were used to amplify a 1,644-bp fragment of the porcine MYOG gene. Primer Sequences. Forward primer: 5¢-TCT ATG ACG GGG AAA ACT AC-3¢; reverse primer: 5¢-TGG AGC CAG AGT GGT GTA TC-3¢.

MyoD and myogenin (Myog) recognize sets of distinct but overlapping target genes and play different roles in skeletal muscle differentiation. MyoD is sufficient for near-full expression of early targets, while Myog can only partially enhance expression of MyoD-initiated late muscle genes. However, the way in which Myog enhances the expression of MyoD-initiated late muscle genes remains unclear....

Angiotensin II (Ang II), an important component of the renin–angiotensin system (RAS), plays a critical role in pathogenesis cardiovascular disorders. In addition, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been considered as promising platform for studying personalized medicine heart diseases. However, whether Ang can induce apoptosis hiPSC-CMs is not known. th...

Skeletal muscle differentiation is mediated by a complex gene expression program requiring both the muscle-specific transcription factor Myogenin (Myog) and p38α MAPK (p38α) signaling. However, the relative contribution of Myog and p38α to the formation of mature myotubes remains unknown. Here, we have uncoupled the activity of Myog from that of p38α to gain insight into the individual roles of...

Muscle inactivity due to injury or disease results in muscle atrophy. The molecular mechanisms contributing to muscle atrophy are poorly understood. However, it is clear that expression of atrophy-related genes, like Atrogin-1 and MuRF-1, are intimately tied to loss of muscle mass. When these atrophy-related genes are knocked out, inactive muscles retain mass. Muscle denervation stimulates musc...

Myogenin (myog) encodes a highly conserved myogenic regulatory factor that is involved in terminal muscle differentiation. It has been shown in mammals that methylation of cytosines within the myog promoter plays a major role in regulating its transcription. In the present study, the Senegalese sole (Solea senegalensis) myog putative proximal promoter was identified and found to be highly conse...

A total of 1,440 fertile eggs were divided randomly and equally into 5 treatment groups; control (no TM) 4 groups: TM1, TM2, TM3, TM4 that thermally subjected to 39°C for 18 h with 65% relative humidity daily, during embryonic days ED 7–11, 11–15, 15–18 7–18, respectively. Out TM conditions investigated, TM1 resulted in a significant improvement expression myogenic factor-5 (Myf5), myoblast det...