introduction: infections by multidrug resistant mycobacterium tuberculosis (mdr-tb) is a major public health challenge. secretion of proteins by m. tuberculosis plays an important role in the pathogenesis of the bacterium. we compared the protein profiles of susceptible m. tuberculosis and mdr-tb isolates using proteomic analyses, namely two dimensional gel electrophoresis (2de) and mass spectr...

Non-tuberculous Mycobacteria (NTM) are emerging as an important opportunistic pathogen. Since clinical presentation of NTM infection is similar to tuberculosis (TB), patients present suspected TB or drug resistant TB. Presently in the National Tuberculosis Elimination Programme (NTEP ) not being speciated, but there urgent need characterize so that appropriate treatment can be given many specie...

Anti genic analysis of mycobacteria by agar-diffusion, first reported by Parlett and Youmans ( 14 ), has been developed by many investigators ( 15, 16, 22) and applied to serologic identification and classification of mycobacteria. Recently, Fukui et aZ. (10) reported that an antigen of M. Zepraemllrium may be identical with the avian y antigen. However, little is known regardin g the antigenic...

The tuberculin skin test for diagnosing tuberculosis (TB) in cattle lacks specificity if animals are sensitized to environmental mycobacteria, as some antigens in purified protein derivative (PPD) prepared from Mycobacterium bovis are present in nonpathogenic mycobacteria. Three immunodominant TB antigens, ESAT6, CFP10, and Rv3615c, are present in members of the pathogenic Mycobacterium tubercu...

Studies were undertaken to find a substance or substances for use in primary binding types of tests to detect humoral antibodies in rabbits and monkeys exposed to the tubercle bacillus that would distinguish between strains of mycobacteria. The antigen employed was a component of the 5159 strain of Mycobacterium tuberculosis that was obtained following sonification, ultracentrifugation, electro...

Freshly isolated human T lymphocytes were tested for their response to mycobacteria, mycobacterial lysates, 2 dimensional (2D) PAGE separated mycobacterial lysates, leishmania and defined leishmanial antigen preparations. While gamma delta T cells proliferated vigorously in the presence of mycobacteria and mycobacteria derived lysates, a significant stimulation from 2 D gel separated lysates wa...

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a s...

Sir, Mycobacteria are known to have several immunologically active antigenic components like 38 Kd antigen, 30 Kd antigen, 16 Kd antigen, A60 antigen and lipoarabinomannan (LAM). 1 The MycoDot test is a new simple, rapid (20 minutes) and reliable serodiagnostic technique that can detect antimycobacterial antibodies in the serum or blood. It offers a low cost, single visit aid in the diagnosis o...

The polymerase chain reaction (PCR) was used to identify mycobacterial DNA sequences in uncultured clinical specimens. Two oligonucleotide primers derived from the sequence of a gene that codes for the 65-kilodalton antigen of Mycobacterium tuberculosis amplified DNA from all 11 species of mycobacteria tested. Amplified DNAs of nontuberculosis mycobacteria were found to be approximately 20 to 4...