conclusions etec and eaggec were the most detected e. coli among stool and uti samples, emphasizing the need to dedicate more health care attention to this group. in addition, our phylogenetic study may be helpful in figuring out the infection origin and for epidemiological studies. nonetheless, more research studies with larger sample sizes are suggested for confirming our results. results ove...

coagulase-positive and coagulase negative, methicillin-resistant staphylococci are major causes of serious nosocomial infections and it is very important to have a reliable test to detect these bacteria. a multiplex polymerase chain reaction (mpcr) was used on 100 clinical samples for simultaneous amplification of the universal bacterial, mec-a encoding the penicillin binding protein 2a, which ...

BACKGROUND & OBJECTIVES Polymerase chain reaction (PCR) has been known to be a rapid and accurate diagnostic test for causative viruses of viral retinitis, but cost is the limiting factor. In the present study an attempt was made to standardize a multiplex PCR (mPCR) on intraocular specimens from patients with viral retinitis for the detection of one or more viruses [herpes simplex virus (HSV),...

INTRODUCTION: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). METHODS: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. RESULTS: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, a...

The kinesin is the main motor protein in the trafficking system of the mitochondria and other organelles. Genetic variants affecting the function of kinesin were earlier found to be associated with cognitive disturbances and neurodegeneration. A multiplex polymerase chain reaction (mPCR) method, allowing the simultaneous detection of the KNS2 A185C (rs_1054080) and C406T (rs_3742465) mutations ...

results using these two sets, we identified h1069q mutation in four patients, c.2335t > g mutation in three, c.3061-1g > a splice site mutation in five, c.3305t > c mutation in one, and c.3809a > g mutation in two patients. conclusions the multiplex arms assay used in this study can be an efficient, reliable, and cost effective method as a primary screen for patients with wilson disease. patien...

BACKGROUND Genetic testing for Duchenne/Becker muscular dystrophy (DMD/BMD) mutations initially involved multiplex polymerase chain reaction (mPCR), which targeted two mutation hotspots in the gene and detected deletions in affected males. A newer technology, multiplex ligation-dependent probe amplification (MLPA), was introduced for diagnostic testing in 2007. OBJECTIVES To evaluate MLPA rel...

background: acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide. objectives: the objective of this study was to characterize antimicrobial resistant diarrheagenic escherichia coli strains isolated from diarrheic children in tehran, ir iran. patients and methods: in total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months...

BACKGROUND Traditionally, the most common diagnostic approach used for diagnosing leptospirosis was the demonstration of immune-seroconversion in acute and convalescent patient serum samples. Recently, a variety of molecular techniques, including conventional and real-time polymerase chain reaction (PCR), have been developed for the specific detection of pathogenic bacteria from the genus Lepto...