Colorectal cancer (CRC) is among deadliest cancers all over the world. Regarding its high mortality, some researches have focused to discover new applicable diagnosis methods. In this regard, circulatory miRNAs has been received huge consideration as promising biomarkers for early detection of CRC. The study aimed to evaluate the expression level of miRNA-7, miRNA-93, and miRNA-409 in plasma of...

Spinal cord injury (SCI) is a catastrophic disease that induces complex cascade of cellular reactions at the local lesion area, including secondary cell death and inflammatory reactions. Accumulating evidence has showed pro- anti-inflammatory roles microRNAs (miRNAs), class small RNAs, in SCI. The present study aimed investigating effects two miRNAs, miRNA-146a miRNA-202-3p, on response after I...

Our understanding of the highly specialized functions for small non-coding single-stranded RNA (ssRNA) in the transcriptome of the human central nervous system (CNS) continues to evolve. Circular RNAs (circRNAs), a recently discovered class of ssRNA enriched in the brain and retina, are extremely stable and intrinsically resilient to degradation by exonuclease. Conventional methods of ssRNA, mi...

The let-7 miRNA (microRNA) is an essential regulator of development from nematode worms to humans. Altered expression of let-7 results in larval arrest or lethality in Caenorhabditis elegans. Likewise, under- or over-expression of let-7 in human cells can result in cellular overproliferation or halted cell division respectively. Thus the biogenesis of this critical miRNA is controlled at multip...

Abstract Background Autism spectrum disorder is a condition related to brain development that affects person’s perception and socialization, resulting in problems social interaction communication. It has no single known cause, yet several different genes appear be involved autism. As genetically complex disease, dysregulation of miRNA expression miRNA–mRNA interactions might feature autism diso...

Circular RNAs (circRNAs) are a naturally occurring family of noncoding RNAs (ncRNAs) highly represented in the eukaryotic transcriptome. Recently characterized, traditional methods of RNA detection and analysis requiring a free 5′ or 3′ ribonucleotide terminus may have significantly underestimated circRNA abundance and significance in eukaryotic cells (Salzman et al., 2012; Wilusz and Sharp, 20...

MicroRNAs (miRNAs) are small RNAs that post-transcriptionally regulate gene expression in many multicellular organisms. They are encoded in the genome and transcribed into primary (pri-) miRNAs before two processing steps that ultimately produce the mature miRNA. In order to generate the appropriate amount of a particular miRNA in the correct location at the correct time, proper regulation of m...

Objective: To verify interaction between miRNA-7 and its target genes, and explore its possible role in schizophrenia. Methods: The hippocampal neuron cells (HT22) in mice transfected by lentiviral vector with microRNA-7 interference and over-expression were cultured, and the microRNA expression profiling of HT22 target genes were measured by quantitative RT-PCR. Results: The expression of thre...

The let-7 microRNA (miRNA) regulates cell cycle exit and terminal differentiation in the C. elegans heterochronic gene pathway. Low expression of let-7 results in retarded vulva and hypodermal cell development in C. elegans and has been associated with several human cancers. Previously, the versatile scaffold protein receptor for activated C kinase 1 (RACK1) was proposed to facilitate recruitme...

BACKGROUND miRNAs are an abundant class of small, endogenous regulatory RNAs. Although it is now appreciated that miRNAs are involved in a broad range of biological processes, relatively little is known about the actual mechanism by which miRNAs downregulate target gene expression. An exploration of which protein cofactors are necessary for a miRNA to downregulate a target gene should reveal mo...