background and objectives : the main cause of serious nosocomial infections is a gram-negative pathogen known as pseudomonas aeruginosa (p. aeruginosa). carbapenems are widely used as an appropriate treatment for these infections, however resistance to these agents has been observed and is increasing. metallo beta-lactamase (mbls) enzyme is one of the main causes of resistance to carbapenem. in...

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Imipenem (N-formimidiyl thienamycin), a carbapenem, is a semisynthetic derivative of thienamycin produced by Streptomyces cattleya. It exerts its bactericidal action by inhibiting cell wall synthesis in aerobic and anaerobic Gram-positive and Gram-negative bacteria. Imipenem shows strong affinity for penicillin binding protein of Escherichia coli and selected strains of Pseudomonas aeruginosa r...

1. Queenan AM, Bush K. Carbapenemases: the versatile β-lactamases. Clin Microbiol Rev. 2007;20:440–58. DOI: 10.1128/ CMR.00001-07 2. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: 20th informational supplement (update 2010 Jun). Document M100–S20-U. Wayne (PA): The Institute; 2010. 3. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, ...

BACKGROUND Continue rise in unprofessional use of antibiotics in our hospitals and communities is worrisome. A research study was therefore conducted to detect extended spectrum beta-lactamase (ESBL), carbapenemase, metallobeta lactamase and their co-production phenotypically from isolates obtained from patients admitted to or attending two tertiary hospitals in Kano, Nigeria. METHOD A total ...

Background and objectives: Metallobeta-lactamase production is one of the most important mechanisms of antibiotic resistance. The aim of the present study was to evaluate the antibacterial activity of Stachys lavandulifolia and Quercus brantii on the IMP-type metallo-beta-lactamase-producing Pseudomonas aeruginosa. Methods: This study...

The Blue-Carba test (BCT) is a biochemical test for rapid ( 2 h) detection of carbapenemase production in Gram-negative bacilli directly from bacterial culture (1). It is based on the in vitro hydrolysis of imipenem by bacterial colonies (direct inoculation without prior lysis), which is detected by changes in pH values revealed by the indicator bromothymol blue (blue to green/yellow or green t...

Klebsiella pneumoniae is the most common causes of pneumonia, urinary tract and bloodstream infections in patients in intensive care unit (ICU). In this study, twenty-two K. pneumoniae cultures were isolated from patients with respiratory tract infections in the same intensive care unit during 2010. All isolates were characterized by XbaI-pulsed-field gel electrophoresis, and the Minimum Inhibi...