pseudomonas aeruginosa is among the most important pathogens in the nosocomial infections. a genetic mobile element, the integron, is one of the major agents involved in dissemination of multi-drug resistance among gram negative bacteria. during a descriptive study from october 2009 to august 2010, some 130 p. aeruginosa clinical isolates were collected from different wards of three hospitals i...

The production of metallo-β-lactamases is the most important strategy by which pathogenic bacteria become resistant to currently known β-lactam antibiotics. The emergence of these enzymes is particularly concerning for the future treatment of bacterial infections. There are no clinically available drugs capable of inhibiting any of the metallo-β-lactamases, so there is an urgent need to find su...

Objective. To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» related to proteins of metallo-β-lactamase group. Materials and Methods. Sequences 7 were analyzed using following resources: ClustalW, IQ-TREE, iTOL. Selection oligonucleotides for real-time PCR was performed Primer-BLAST resource. The level gene expression assessed PCR. MIC MBC me...

background and objectives: pseudomonas aeruginosa is a frequent opportunistic pathogen in health care associated infections that is highly resistant to the majority of β-lactams. the aims of this study were to access the antimicrobial susceptibility pattern of p. aeruginosa isolated from educational hospitals of qazvin and alborz provinces, to determine the prevalence of metallo-β-lactamase (mb...

background: resistant strains of pseudomonas aeruginosa to imipenem was medical treatment problem, especiallyin burnt units of hospitals. objectives: this study was conducted to evaluate the antimicrobial effect of fe2o3 nanoparticles alone and functionalized with imipenem on p. aeruginosa starins producing metallo b-lactamases (mbl).materials and methods: a disk diffusion method was used to is...

conclusions this study showed that multiple β-lactamases can be produced in burn isolates. this suggests that use of cephalosporins and carbapenems should be restricted in burn isolates to minimize the development and spread of these multidrug resistant pathogens. results there was 99% resistance to carbenicillin, and ticarcillin, 98% to cotrimoxazole, 96% to ciprofloxacin, and aztreonam, 95% t...

BACKGROUND Metallo-β-lactamases are bacterial enzymes that provide resistance to carbapenems, the most potent class of antibiotics. These enzymes are commonly encoded on mobile genetic elements, which, together with their broad substrate spectrum and lack of clinically useful inhibitors, make them a particularly problematic class of antibiotic resistance determinants. We hypothesized that there...

Metallo-β-lactamase-producing Pseudomonas aeruginosa (MPPA) is an important nosocomial pathogen that shows resistance to all β-lactam antibiotics except monobactams. There are various types of metallo-β-lactamases (MBLs) in carbapenem-resistant P. aeruginosa including Imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), Sao Paulo metallo-β-lactamase (SPM), Germany imipenemase (...

conclusions the results of this study reveal that the m. communis extract and methanolic extract of chamomile have a high antibacterial effect on regular and imp-producing p. aeruginosa strains; so, these extracts can be suitable alternatives for less-effective antibiotics, which are commonly used. materials and methods this cross-sectional descriptive study was conducted on burn patients hospi...

INTRODUCTION Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of Sergipe, Brazil. METHODS Metallo-β-lactamase was investigated using the disk approximation test...