نتایج جستجو برای: mediated isothermal amplification (lamp)

تعداد نتایج: 480656  

Journal: :iranian journal of microbiology 0
reza ranjbar molecular biology research center, baqiyatallah university of medical sciences, tehran, iran. davoud afshar molecular biology research center, baqiyatallah university of medical sciences, tehran, iran.

background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...

Journal: :iranian journal of virology 0
b pourhossein department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran h soleimanjahi department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran f behzadian molecular genetics department, research center for sciences and biotechnology, tehran, iran b khansarinejad department of virology, faculty of medical sciences, tarbiat modares university, tehran, iran

background and aims: considering difficulties in usual laboratory methods in detection of viral infections, improved dna-based diagnostic techniques are more reliable. loop mediated isothermal amplification method (lamp) is a nucleic acid amplification method that amplifies dna using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. in this study, the...

احمدی, زینب, جوادی, سیده مهری, حق نظری, علی, سروری زنجانی, رحیم, مرادی, ابوبکر, کرمی, علی,

Background and Objective: There are several techniques for the diagnosing of salmonella infectious. Several molecular methods such as PCR and hybridization assay have recently been used for the detection of this bacterium. However, these methods require precision instruments for amplification and complex procedures, which are the major obstacles to the widespread use of these methods in relativ...

2012
Nelson R. Salinas Damon P. Little

We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole ...

Journal: :Journal of clinical microbiology 2014
Kelly A Curtis Philip L Niedzwiedz Ae S Youngpairoj Donna L Rudolph S Michele Owen

Currently, there are no FDA-approved nucleic acid amplification tests (NAATs) for the detection or confirmation of HIV-2 infection. Here, we describe the development of a real-time assay for the detection of HIV-2 DNA and RNA using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isothermal amplification/detection device.

Journal: :Veterinaria italiana 2015
Mayurkumar Bhimani Bharat Bhanderi Ashish Roy

Twenty two isolates of Pasteurella multocida were obtained from different tissues of dead birds and animals (cattle, buffalo, sheep, and goat) suspected of fowl cholera and haemorrhagic septicaemia. The isolates were confirmed as P. multocida by various biochemical tests and PM PCR. An attempt was made to standardize Loop mediated isothermal amplification (LAMP) using newly designed primer sequ...

ژورنال: :مجله دانشگاه علوم پزشکی مازندران 0
آزاده میزانی azadeh mizani 1 phd student in medical parasitology, toxoplasmosis research center, mazandaran university of medical sciences, sari, iran 2 phd student in medical parasitology, student research committee, mazandaran university of medical sciences, sari, iranدانشجوی دکتری انگل شناسی پزشکی، مرکز تحقیقات توکسوپلاسموز، دانشگاه علوم پزشکی مازندران، ساری، ایران 2. دانشجوی دکتری انگل شناسی پزشکی، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی مازندران، ساری، ایران پوریا گیل pooria gill assistant professor, department of physiology, pharmacology and nanotechnology, mazandaran university of medical sciences, sari, iranدانشیار، گروه فیزیولوژی، فارماکولوژی و نانوتکنولوژی، دانشگاه علوم پزشکی مازندران، ساری، ایران شهاب الدین سروی shahabeddin sarvi assistant professor, toxoplasmosis research center, mazandaran university of medical sciences, sari, iranساری: کیلومتر 18 جاده فرح آباد، مجتمع دانشگاهی پیامبر اعظم، دانشکده پزشکی احمد دریانی ahmad daryani professor, toxoplasmosis research center, mazandaran university of medical sciences, sari, iranاستاد، مرکز تحقیقات توکسوپلاسموز، دانشگاه علوم پزشکی مازندران، ساری، ایران مهدی شریف mehdi sharif professor, toxoplasmosis research center, mazandaran university of medical sciences, sari, iranاستاد، مرکز تحقیقات توکسوپلاسموز، دانشگاه علوم پزشکی مازندران، ساری، ایران افسانه عمویی afsaneh amouei phd student in medical parasitology, student research committee, mazandaran university of medical sciences, sari, iranدانشجوی دکتری انگل شناسی پزشکی، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی مازندران، ساری، ایران علی باکویی کتریمی

سابقه و هدف: فاسیولا هپاتیکا و فاسیولا ژیگانتیکا فلوک های کبدی شایعی بوده که روی انسان ها و دام ها در تمام نقاط جهان اثرگذار هستند. در مطالعه حاضر ما تکنیک سریع loop-mediated isothermal amplification (lamp) را در تشخیص و افتراق گونه های فاسیولا ارزیابی نمودیم. مواد و روش ها: تعداد 50 کرم فاسیولا از کبد گاو و گوسفند کشتارگاه های استان مازندران جداسازی شدند. تعداد 8 پرایمر جهت تکثیر ناحیه ژنی 28s ...

Journal: :jundishapur journal of microbiology 0
somaye bakhtiari department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran amirhooshang alvandi department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran hamid pajavand department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran jafar navabi imam khomeini hospital, kermanshah university of medical sciences, kermanshah, ir iran farid najafi department of biostatistics and epidemiology, school of hygiene, kermanshah university of medical sciences, kermanshah, ir iran ramin abiri department of microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran; department of microbiology, school of medicine, kermanshah university of medical sciences, p. o. box: 6714869914, kermanshah, ir iran. tel: +98-9122773648, fax: +98-8314274623

conclusions the analytical sensitivity of lamp and pcr methods, using the designed primers, was 8 times more than any other reported methods. the designed methods are specific and sensitive for detection of h. pylori in different clinical and environmental samples. results the detection limits of the lamp and pcr assay were similar and were 10 fg of pure dna of h. pylori, which is equal to 6 co...

Journal: :BioTechniques 2015
Nathan A Tanner Yinhua Zhang Thomas C Evans

Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both. Here we present a novel method of amplification detection that harnesses the pH change resulting from amplification reactions performed with minimal buffering capacity....

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید