Human granulocyte macrophage colony stimulating factor (hGM-CSF) has many therapeutic applications.In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration andpost-induction time on the secretion of recombinant hGM-CSF into the culture medium by recombinantEscherichia coli during high cell density cultivation were evaluated by...

fusion of two genes at dna level produces a single protein, known as a chimeric protein. immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. in this investigation, the catalytic domain of shiga-like toxin (a1) was fused to human granulocyte macrophage c...

a self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pl promoter, was constructed. the designed 5024 base pair expression plasmid contained a heat sensitive repressor ci857 coding gene to regulate the function of ?pl promoter under heat shock induction. using the constructed vector,...

A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...

With the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hGM-CSF) in Escherichia coli, hGM-CSF cDNA was fused in-frame next to the signal sequence of ST toxin (ST-I) of exteroxigenic E. coli, containing 53 or 19 amino acids of signal peptide. The fused STsig::hGM-CSF coding fragments were inserted into a T7-based expression plasmid. The recombinant plasmids were ...

the cytokine of granulocyte macrophage colony stimulating factor (gm-csf) is a glycoprotein, which is synthesized in the female reproductive tract and has embryonic trophic effect in mammals. the objective of this study was to examine the optimal dosage of gm-csf to improve the mouse embryo development in vitro. to collect two and eight cells embryos, the pregnant nmri mice were sacrificed by c...

with the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hgm-csf) in escherichia coli, hgm-csf cdna was fused in-frame next to the signal sequence of st toxin (st-i) of exteroxigenic e. coli, containing 53 or 19 amino acids of signal peptide. the fused stsig::hgm-csf coding fragments were inserted into a t7-based expression plasmid. the recombinant plasmids were ...

Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human granulocyte macrophage colony-stimulating factor (hGM-CSF), which gets its name from the Bm5-hGM-CSF cell in which the glycoprotein of the hGM-CSF is secreted in the cell culture supernatant (CCS). It was demonstrated that secreted hGM-CSF had in vivo biological activity and the white blood cell (WBC) v...

purpose: in order to express human granulocyte-macrophage colony stimulating factor (hgm-csf) under heat shock. materials and methods: two expression plasmids were constructed based on pbc(sk) plasmid. the expression cassettes in the two plasmids are equipped with a 75 base pair fragment, derived from the pl promoter of the bacteriophage lambda (λ). the plasmids also contain a temperature muta...

Bacterial expression systems provide economic and logistic advantages in manufacturing proteins for human therapeutic purposes. However, most such proteins accumulate in insoluble biologicaIIy inactive form when overexpressed in bacterial cells. This is the case while attempting to produce recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) in E. coli. In this repor...