foot-and-mouth disease (fmd) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. this study quantifies the cytokines mrna expression of vaccinated guinea pigs withfmd type o inactivated vaccine. blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. extracted mrnas were reverse-transcribed into cdna and analyz...

the mrna expression of growth hormone (gh) gene in early development stages of siberian sturgeon was investigated using rt-pcr method. samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. ribosomal protein l6 (rpl6) transcripts were used as the internal standard during quantification of gh mrna expression. the results showed that the gh mr...

background: recently, reports have indicated a role for the membrane form of toll-like receptor 2 (tlr2) in asthma pathogenesis. in this study we examined soluble tlr2 levels in serum and sputum of asthmatic and healthy subjects. methods: serum and sputum samples were obtained from 33 asthmatic and 19 healthy subjects. the asthmatics were classified into four groups according to the global init...

bakground: multiple sclerosis (ms) is a cd4+ t cell-mediated autoimmune disease affecting the central nervous system (cns). it was previously believed that th1 cells were pathogenic t cells in experimental autoimmune encephalomyelitis (eae). however, the functional role of th1 cells in eae has been reconsidered upon the discovery of il-17- producing t cells which are consider as dominant effect...

conclusions the obtained results indicate that rsm is an effective method for the optimization of culture conditions for hes gene expression in e. coli. results a maximum rhes mrna level of 376.16% was obtained under the following conditions: 0.6 mm iptg, 7 hours post-induction time, and 0.9 cell density before induction. the level of rhes mrna was significantly correlated with post-induction t...

Objective PCOS is the most frequent female endocrine disorder, affecting 5%-10% of women, is characterized by hyperandrogenism, oligo-/anovulation, and polycystic ovaries. The aim of the present research is to evaluate the expression of steroidogenic acute regulatory protein (StAR) and CYP19 (aromatase) mRNA in the ovary of EV-induced PCOS rat model and the effect of the treadmill and running w...

Background: Acute lymphoblastic leukemia (ALL) results from genetic alterations in a single lymphoid progenitor cell. Expression of ROR1 is reported to be increased in ALL and mantle cell lymphoma. In this study the expression of ROR1 was assessed in newly diagnosed patients with ALL.  Methods: This study was carried out on 40 patients with newly diagnosed ALL and healthy individuals as contro...

conclusions further studies on the role of hepcidin in pathogenesis of chc are needed to assess the potency of its use in antiviral treatment. results patients with chc had lower hepcidin mrna expression and more frequently iron deposits in hepatocytes than subjects with chb did. in chc group, hepcidin mrna expression was positively correlated with alanine aminotransferase activity and serum ir...

background sall gene family represent a group of evolutionary conserved zinc finger transcription factors which are involved in normal development. it includes four members (sall1 to sall4). sall4 has significant roles in the maintenance of pluripotency and self-renewal, efficient proliferation /stabilization and cell fate decision of embryonic stem cells (escs). our aim in this study was to an...

Background SALL gene family represent a group of evolutionary conserved zinc finger transcription factors which are involved in normal development. It includes four members (SALL1 to SALL4). SALL4 has significant roles in the maintenance of pluripotency and self-renewal, efficient proliferation /stabilization and cell fate decision of embryonic stem cells (ESCs). Our aim in this study was to a...