The reproductive potential of Meloidogyne graminicola was compared with that of M. incognita on Trifolium species in greenhouse studies. Twenty-five Trifolium plant introductions, cultivars, or populations representing 23 species were evaluated for nematode reproduction and root galling 45 days after inoculation with 3,000 eggs of M. graminicola or M. incognita. Root galling and egg production ...

A de novo search for repetitive elements in the genome sequence of the wheat pathogen Mycosphaerella graminicola identified a family of repeats containing a DNA cytosine methyltransferase sequence (MgDNMT). All 23 MgDNMT sequences identified carried signatures of repeat induced point mutation (RIP). All copies were subtelomeric in location except for one on chromosome 6. Synteny with M. fijiens...

ABSTRACT The frequency of sexual reproduction has a profound effect on the population structure and the adaptive potential of a facultatively sexual parasite. Little is known about the relationship of quantitative host resistance to the frequency of sex in pathogens. We sampled over 5,000 fungal fruiting bodies from eight different wheat cultivars over a 3-year period. The cultivars possessed v...

A simple and applicable technique was developed for the detection of soil infestation with rice rootknot nematode, Meloidogyne graminicola by farmers themselves at their fields. The collected soil samples from root-knot nematode infested fields were diluted by adding sterilized sand in the ratio of 1: 1, 1: 3, 1: 7, 1: 15, 1: 31 and 1: 63 and sown with sprouted rice seeds (cv MUT 7029) for dete...

Medical drugs known to modulate the activity of human ATP-binding cassette (ABC) transporter proteins (modulators) were tested for the ability to potentiate the activity of the azole fungicide cyproconazole against in vitro growth of Mycosphaerella graminicola and to control disease development due to this pathogen on wheat seedlings. In vitro modulation of cyproconazole activity could be demon...

Early detection of infection is very important for efficient management of Mycosphaerella graminicola leaf blotch. To monitor and quantify the occurrence of this fungus during the growing season, a diagnostic method based on real-time PCR was developed. Standard and real-time PCR assays were developed using SYBR Green chemistry to quantify M. graminicola in vitro or in wheat samples. Microsatel...

The fungus Mycosphaerella graminicola has been a pathogen of wheat since host domestication 10,000-12,000 years ago in the Fertile Crescent. The wheat-infecting lineage emerged from closely related Mycosphaerella pathogens infecting wild grasses. We use a comparative genomics approach to assess how the process of host specialization affected the genome structure of M. graminicola since divergen...

A wild species of jute, Corchorus aestuans (family: Tiliaceae), was found to be infected root-knot nematode, Meloidogyne graminicola. The pathogenicity M. graminicola on C. (local cultivar) under pot conditions showed that could infect, reproduce, and incite profuse root galling in aestuans. initial inoculum 10-second stage juveniles per 1000 cm3 soil displayed the highest reproduction factor (...

The plant-pathogenic fungus Mycosphaerella graminicola (asexual stage: Septoria tritici) causes septoria tritici blotch, a disease that greatly reduces the yield and quality of wheat. This disease is economically important in most wheat-growing areas worldwide and threatens global food production. Control of the disease has been hampered by a limited understanding of the genetic and biochemical...

Meloidogyne graminicola is one of the most economically important plant parasitic-nematodes (PPNs). In the present study, we determined the complete mitochondrial (mt) DNA genome sequence of this plant pathogen. Compared with other PPNs genera, this genome (19,589 bp) is only slightly smaller than that of Pratylenchus vulnus (21,656 bp). The nucleotide composition of the whole mtDNA sequence of...