Vancomycin monotherapy of deep-seated staphylococcal infection may be associated with poor bacteriological response. We evaluated 24 unique patient isolates of methicillin-resistant Staphylococcus aureus (MRSA) for vancomycin-gentamicin synergism by determining time-kill curves for vancomycin at 10 micrograms/ml and gentamicin at 1 microgram/ml. Nine MRSA strains showed high-level gentamicin re...

The effects of teicoplanin (8 micrograms/ml), ampicillin (64 micrograms/ml), imipenem (32 micrograms/ml), and gentamicin (4 micrograms/ml), alone and in combination, against 13 unique blood isolates of vancomycin-resistance (MIC for 90% of isolates tested [MIC90], 512 micrograms/ml), teicoplanin-susceptible (MIC90, 2.0 micrograms/ml), ampicillin-resistant (MIC90, 128 micrograms/ml), and non-bet...

Streptococcus agalactiae (group B Streptococcus [GBS]) is a leading cause of sepsis in neonates. The rate of invasive GBS disease in nonpregnant adults also continues to climb. Aminoglycosides alone have little or no effect on GBS, but synergistic killing with penicillin has been shown in vitro. High-level gentamicin resistance (HLGR) in GBS isolates, however, leads to the loss of a synergistic...

27 Streptococcus agalactiae (group B Streptococcus, GBS) is a leading cause of sepsis in 28 neonates. The rate of invasive GBS disease in non-pregnant adults also continues to climb. 29 Aminoglycosides alone have little or no effect on GBS, but synergistic killing with penicillin 30 has been shown in vitro. High-level gentamicin resistance (HLGR) in GBS isolates, however, 31 leads to loss of a ...

BACKGROUND Cold atmospheric plasma could constitute an alternative against multi-drug resistant pathogens. Susceptibility of enterococci to cold atmospheric plasma was investigated in vitro. METHODS 39 clinical isolates of enterococci were grouped dependent on the most important resistance patterns and treated on agar using dielectric barrier discharge plasma. These included enterococci with ...

BACKGROUND During the last decade, enterococci have become important nosocomial pathogens, representing the second leading cause of urinary tract infections. This increasing prevalence has been paralleled by the occurrence of multi-drug resistant (MDR) and high-level gentamicin resistant (HLGR) strains. METHODS From September 2005 to 2006, a total of 638 enterococcal isolates were collected f...

We determined the species distribution and prevalence of ampicillin resistance, high-level gentamicin resistance (HLGR) and vancomycin resistance among clinical enterococcal isolates from five Nordic laboratories (Bergen, Tromsø, Uppsala, Aarhus and Reykjavik). Isolates represented three different groups: (i) all blood culture isolates from 1999; (ii) consecutive in-patient isolates (maximum 40...

background and objective: high-level gentamicin resistance (hlgr: mic ≥ 500 µg/ml) in enterococci is mediated by aminoglycoside modifying enzymes which is mainly encoded by aac(6′)-ie-aph(2′′)-ia gene. the aim of this study was to evaluate the frequency of   aac(6′)-ie-aph(2′′)-ia gene in clinical isolates of enterococcus facium and enterococcus faecalis collected from hospitals in northwest of...

High-level gentamicin resistance (HLGR) in enterococci has increased since the 1980s, but the clinical significance of the resistance and its impact on outcome have not been established. One hundred and thirty-six patients with bacteremia caused by enterococci with HLGR (HLGR group) were compared with 79 patients with bacteremia caused by enterococci without HLGR (non-HLGR group). Hematologic m...

background: enterococci are members of the normal gut flora and released into the environment via sewage outlets, where they can survive for long times. infections with high-level gentamicin resistant (hlgr) enterococci are emerg­ing worldwide. hlgr enterococci have developed a resistance to most antibiotics commonly used for enterococcal in­fec­tions therefore; treatment of infections caused b...