different examination have shown that rheumatoid factor is not responsible for false positive reaction (f.p.r.) in cr. neoformanse’s free capsular antigen latex agglutination test, whereas high levels of iron in sera of patients with rheumatoid arthritis as well as other sera was responsible not only for this f.p.r. , but also had an important role in the production of f.p.r. in many slide late...

in this study which has been carried out from 1996 until 1997 soluble latex agglutination antigen was prepared from L, donovani,sudan(s-1) by culturing in special media,harvesting,and later solubilizing by freezing and thawing.the antigen were futher prepared by high speed centrifugation.after measurement of the protein (lowry method) concentration of the antigen was set at 2.2 mg/ml,the antige...

The purpose of this research was to develop a simple and rapid diagnostic test for scrub typhus using a latex agglutination test (LAT) to detect antibodies against Orientia tsutsugamushi. Five strains of O. tsutsugamushi were propagated in L929 cells. The rickettsiae were purified and concentrated with percoll density gradient centrifugation. A suitable concentration of O. tsutsugamushi soluble...

The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect...

A latex agglutination test (LAT) based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the hemagglutination inhibition test, the sensitivity and specificity of the LAT were 88.8 and 97.6%, respectively, in detecting 830 serum samples from vaccinated chickens. The test has application potential in field practice.

A therapeutic anti-rabies immunoglobulin for human use has been produced mainly in horses. The presently available seroneutralization test, the rapid fluorescent focus inhibition test (RFFIT), is laborious and rather difficult to carry out in horse farms. This study was undertaken to develop a simple latex agglutination test (LAT) for determining rabies antibodies in horse sera. LAT was validat...

In this study, sLPS antigen coated latex bead was prepared and their evaluation was done along with RBPT, commonly employed for the serodiagnosis of brucellosis. sLPS was extracted from Brucella abortus S99 and coated on polystyrene latex beads in a phosphate buffer saline coating buffer. A total of 200 samples were collected from unorganized dairy farm and tested with RBPT and sLPS based latex...

Applicability and sensitivity of the commercial tests MAT (modified agglutination test), LAT (latex agglutination test), in comparison to in-house serologic assay (ELISA), and reference test (indirect fluorescence antibody test, IFAT) to detect specific antibodies against Toxoplasma gondii in pigs were investigated. One hundred and six blood samples were examined. The most specific test, as com...

A rapid whole cell protein antigen (iron restricted Mycoplasma gallisepticum) based latex agglutination test (LAT) was developed to detect the presence of antiMycoplasma gallisepticum antibodies in the sera of poultry affected with mycoplasmosis. The test was found to be sensitive, specific and accurate in comparison with Haemagglutination Inhibition (HI) test. The rapidity, simplicity and econ...

Background: Rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. Since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included Electron Microscopy (EM) and enzyme immunoassay. These methods, however, are expensive and not readily available everywhere. We have developed a technique which can be used for ...