The isolation of K99 Escherichia coli (K99 E. coli) was performed using diarrheal stool which occurred in calves from january through October, 1988. Moreover, bacteriolgical examination of isolated K99 E. coli and the adherent condition of K99 antigen in the intestinal canal of a dead calf was observed. In addition an epidemiological survey mainly including antibody examination of adult cattle ...

Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carri...

The effects of a maternal vaccine against newborn calf diarrhea associated with group A bovine rotavirus (BRV), bovine coronavirus (BCV), bovine parvovirus and K99 Escherichia coli (E. coli) were examined on a beef cow-calf herd. After vaccination, serum or colostrum antibody titers to BRV, BCV and E. coli K99 in the vaccinated cows were significantly higher than those in unvaccinated control c...

The isoelectric point of the K99 antigen in partially purified preparations isolated from Escherichia coli B41 was 4.2. Electrofocused K99 antigen hemagglutinated guinea pig and sheep erythrocytes and gave a single precipitin line on diffusion against antisera to E. coli B41 and absorbed factor K99 antisera.

Pregnant cattle were either vaccinated subcutaneously with (i) a suspension of purified Escherichia coli K99 pili, (ii) a Formalin-killed whole cell bacterin containing enterotoxigenic E. coli strain B44 (O9:K30;K99:H-), or (iii) a bacterin containing six different strains of bovine enterotoxigenic E. coli (multiple-strain bacterin), or were left as nonvaccinated controls. After birth, calves w...

OBJECTIVE We determined whether Gram-negative bacterial molecules are associated with Alzheimer disease (AD) neuropathology given that previous studies demonstrate Gram-negative Escherichia coli bacteria can form extracellular amyloid and Gram-negative bacteria have been reported as the predominant bacteria found in normal human brains. METHODS Brain samples from gray and white matter were st...

Enterotoxigenic Escherichia coli (ETEC) produces different virulence factors allowing the bacterium to colonize and develop watery diarrhea. Proteomics studies have also introduced new protein belonging to the secretion pathways, antigen 43 (Ag43), which plays important role in E. coli pathogenesis. The objective of this study was to investigate O-types and virulence factors o...