Background: Polarized cells are key to the process of differentiation. Xenopus oocyte is a polarized cell that has complete blue-print to differentiate 3 germ layers following fertilization, as key determinant molecules (Proteins and RNAs) are asymmetrically localized. The objective of this work was to localize Na+, K+-ATPase activity along animal-vegetal axis of polarized Xenopus oocyte and fo...

the ultrastructure of the cells, na+, k+-atpase activity and immunolocalization were examined in the barnchial chamber of libellula lydia (drury, 1773) larvae. na+,k+-atpase activity and localization were performed through biochemical techniques and immunofluorescence light microscopy using a mouse monoclonal antibody iggα5, respectively. the branchial chamber possesses six pair gills lamellae ...

introduction: the regulation of the body fluid content (osmoregulation) of an aquatic animal, is performed by several organs. in fish, osmoregulatory mechanisms are based on the function of specialized cells (ionocytes) located in various tissues and organs including gills. na+, k+-atpase is one of the main osmoregulatory enzymes enabling the use of atp as a source of energy for ion transport t...

macrobrachium rosenbergii lifecycle is unique so that the adults thrive in freshwater and larval development requires brackish water environments near to estuaries as close as possible to larval body isosmotic for minimum na/k-atpase activity and maximum atp storage. osmoregulation system at early larval stages is not formed and larval osmoregulation is mainly attributed to na/k-atpase activity...

objective: changes in chloride cell abundance, na+, k+-atpase immunolocalization and activity were investigated in the gills of the golden grey mullet, liza aurata, fry acclimated to freshwater (fw) and different salinities (12‰, 36‰ and 46‰). materials and methods: na+, k+-atpase localization was performed through immunofluorescence light microscopy using a mouse monoclonal antibody iggα5. qua...

objective: the urinary system of euryhaline fish, as well as the gills, is involved in ion regulation through the production of dilute urine in freshwater and isotonic urine in seawater. the low osmolality of the urine originates from an active reabsorption of ions by ionocytes or mitochondria rich-cells (mrcs) present in certain parts of the urinary system. mitochondria rich-cells possess a hi...

this study was carried out to determine the appropriate size of caspian trout ( salmo caspius kessler, 1877) juveniles for releasing into the rivers that enter south of the caspian sea for stock enhancement, or possibility of cage culture in the caspian sea. a total of 1611 specimens in 4 weight groups of 5, 10, 15 and 20g were exposed to 3 salinity trials which include caspian sea water (11- 1...